This article comprises detailed information about L-asparaginase, encompassing topics such as microbial and plant sources of L-asparaginase, treatment with L-asparaginase, mechanism of action of L-asparaginase, production, purification, properties, expression and characteristics of l-asparaginase along with information about studies on the structure of L-asparaginase. Although L-asparaginase has been reviewed by Savitri and Azmi (2003), our effort has been to include recent and updated information about the enzyme covering new aspects such as structural modification and immobilization of L-asparaginase, recombinant L-asparaginase, resistance to L-asparaginase, methods of assay of L-asparagine and L-asparaginase activity using the biosensor approach, L-asparaginase activity in soil and the factors affecting it. Also, side-effects of L-asparaginase treatment in acute lymphoblastic leukemia (ALL) have been discussed in the current review. L-asparaginase has been and is still one of the most widely studied therapeutic enzymes by researchers and scientists worldwide.

The present work aims at the development of a novel, diagnostic biosensor for monitoring asparagine levels in leukemia. Various immobilization strategies have been applied to improve the stability of the biocomponent (asparaginase). Response time studies have been carried out for different immobilization methods. Phenol Red indicator has been coimmobilized with asparaginase and color visualization approach has been optimized for various asparagine ranges. The detection limit of asparagine achieved with nitrocellulose membrane is 10(-1) M, with silicon gel is 10(-10)-10(-1) M, and with calcium alginate beads is 10(-9)-10(-1) M. Furthermore, the calcium alginate bead system of immobilization has been applied for the asparagine range detection in normal and leukemia serum samples.

No abstract

No abstract

The purpose of this study was to determine the relationship between biochemical markers such as serum calcium (Ca), phosphorus (P), intact parathyroid hormone (iPTH), 25(OH) vitamin D, and fibroblast growth factor 23 (FGF23) in our study group, as well as to correlate dual-energy X-ray absorptiometry (DEXA) findings with these biochemical markers. MethodologyAn eligible group of 50 chronic hemodialysis (HD) patients, age 18 and older, who have undergone HD two times a week for at least six months participated in this retrospective cross-sectional study. We compared serum FGF23, intact parathyroid hormone (iPTH), 25(OH) vitamin D, calcium, phosphorus, and dual-energy X-ray absorptiometry scan showing bone mineral density disorder (BMD) around the femoral neck, distal radius, and lumbar spine. Human FGF23 Enzyme Linked Immuno Sorbent Assay (ELISA) Kit PicoKine® (Catalog # EK0759; Boster Biological Technology, Pleasanton, CA) was used in the optimum moisture content (OMC) lab to measure FGF23 levels. For the analysis of associations with various studied variables, the levels of FGF23 were split into two groups, which were high (group 1, FGF23 50 to 500 pg/ml), that is, up to 10 times the normal levels and extremely high (group 2, FGF23 > 500 pg/ml) FGF23 levels. All the tests were conducted for routine examination where the data obtained was analyzed in this research project.

No abstract

The topic of this case study is Clash of ideas and sensibility in writings of Joseph Conrad and Chinua Achebe. I have portrayed how both the authors have represented Africa in their writings. Joseph Conrad is a European whereas Chinua Achebe is an African. Both of them have contrary views about the people of Africa and Africa as a country. Both show some of the effects that the white colonists had in the area, and the influence they had on the natives. In Heart of Darkness, we see the influence of Kurtz over the natives at the Inner Station, where they revered him almost as a god. At the other stations, we also see the natives being affected by the white colonists, changing their ways of living around the station, and following what the white men's command, for the most part. In Things Fall Apart, we see this in Okonkwo's home village, where the white colonists set up a District Commissioner (D.C), and the natives bend to the laws he sets, even helping him enforce them. This completely changes their previous way of life.

Friends, nowadays the level of water is decreasing day by day, to save this level of water, all the people are making efforts such as collecting rain water, using RO’s waste water for plants etc. We also wanted to make an effort which will help to a great extent in saving the water level. Friends, you must have seen that 10 liters of water is stored in the flush tank and using it repeatedly consumes a lot of water. To save this water, we have thought of a solution. It will have an arrangement in which two containers of 2 liter and 5 liter will be placed inside the flush tank. The waste water from the kitchen sink will be sent to the sewerage which will be stored in the storage tank and purified and reused in the flush tank. This idea will be of great benefit.