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This study was designed to investigate alterations in coagulation, and in biochemical and haematological parameters in cattle with traumatic reticuloperitonitis (TRP). In the study, 28 dairy cattle with TRP and 10 clinically healthy cattle (control) of different ages and breeds were used. Cattle with TRP had prolonged prothrombin time (PT), thrombin time (TT) and activated partial thromboplastin time (APTT). Erythrocytopenia, thrombocytopenia and hyperfibrinogenaemia were detected in animals with TRP. Furthermore, the serum concentrations of total protein, globulin and total bilirubin, and the activities of alkaline phosphatase (ALP) and aspartate aminotransferase (AST) were also high in cattle with TRP compared to those of the control group. The serum concentrations of calcium were significantly low in the TRP group. The results of this study, therefore, indicate that TRP causes significant coagulation abnormalities and biochemical and haematological alterations in dairy cattle.

This study aims to determine how neopterin, procalcitonin, biochemical and hematological parameters change during treatment of calves with neonatal sepsis. A total of 25 calves divided into two groups. Sepsis group was composed of 15 newborn calves aged 0–10 days which met neonatal sepsis criteria, but did not receive any treatment. Control group included 10 healthy calves aged 0–10 days. Clinical examinations (respiratory rate, rectal temperature, heart rate, capillary refill time, sucking reflex) were performed at certain times before (0th h) and during (12th, 24th, 48th, and 72th h) the treatment. The blood was taken from the jugular vein from the sepsis group before (0th h) and during the treatment (12th, 24th, 48th, and 72nd h) and once from the control group. Procalcitonin pretreatment (0th h) and control group concentrations were found as 178.08 ± 2.4 (pg/mL) and 42.78 ± 1.25 (pg/mL), respectively ( p < 0.001). Neopterin pretreatment (0th h) and control group concentrations were determined as 14.44 ± 0.30 (ng/mL) and 3.63 ± 0.29 (ng/mL), respectively ( p < 0.001). As a result, neopterin and procalcitonin concentration decreased along with the treatment, confirming the presence of sepsis in calves and suggesting that sepsis could be a prognostic indicator. Therefore, both procalcitonin and neopterin can be prognostic and diagnostic in calves with sepsis.

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Aim:The aim of this study was to determine the alterations in the lipid profile, plasma alkaline phosphatase (ALP) activity, total and direct bilirubin levels of neonatal calves with diarrhea.Materials and Methods:A total of 25 calves with diarrhea as experimental group and 10 healthy calves as control group, 1-30 days old, were enrolled in the study. Blood samples were collected from jugular vein in tubes with anticoagulant agent to evaluate the concentration of triglycerides, total cholesterol, high-density lipoprotein-cholesterol (HDL-C), ALP, total and direct bilirubin. Very low-density lipoprotein-cholesterol (VLDL-C) and low-density lipoprotein-cholesterol (LDL-C) levels were calculated according to the Friedewald formula.Results:Significant increases in the plasma levels of ALP (p<0.05), total and direct bilirubin, triglycerides, and VLDL-C (p<0.01) were determined, whereas significant decreases in the levels of total cholesterol, HDL-C and LDL-C (p<0.01) were observed in neonatal calves with diarrhea.Conclusion:According to the findings of this study, liver functions impaired and, therefore, lipid profile is affected negatively in neonatal calves with diarrhea.

Aim:In this study, it was aimed to determine the concentration of some important acute phase proteins (APPs) and some biochemical parameters pre-operative and post-operative in calves with omphalitis.Materials and Methods:A total of 20 calves were used in the study and they consist of 10 clinically healthy calves that were used as a control and 10 calves with omphalitis were used as the treatment group. Blood samples were collected from Vena jugularis of animals to tubes with anticoagulant (sodium citrate) and without anticoagulants, pre-operative (day 0), and post-operative (day 7). Samples were used to determine the concentration of haptoglobin (Hp), serum amyloid A (SAA), ceruloplasmin (Cp), fibrinogen, glucose, total protein, albumin, urea, total bilirubin, creatinine, calcium, phosphorus, aspartate aminotransferase (AST), alkaline phosphatase (ALP), and gamma-glutamyl transferase (GGT) concentrations.Results:While the Hp, SAA, Cp, fibrinogen, urea, creatinine, total bilirubin, ALP, and GGT concentrations were statistically and significantly increasing rather than the control group during the pre-operative period for calves with omphalitis, they decreased to the post-operative period. Moreover, an insignificant increase in the glucose, total protein, and AST concentrations and an insignificant decrease in the albumin, calcium, and phosphorus concentrations were statistically determined.Conclusion:We have the opinion that the assessment of biochemical parameters and especially APP levels in calves with the omphalitis together with the clinical findings may be important in terms of the treatment and prognosis.

ABSTRACT. The purpose of the present study was to determine concentrations of some biochemical parameters and oxidative stress levels in cattle with brucellosis. For this purpose, a study group included with 20 cattle with brucellosis and a control group with 10 clinically healthy cattle were used. Blood samples were collected into the tubes (with and without anticoagulant agent) from the Jugular vein of animals in each group. The reduced glutathione (GSH) in whole blood and levels of malondialdehyde (MDA) and nitric oxide (NO) were determined spectrophotometrically. Additionally, aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea, creatinine and iron (Fe) levels in serum samples were colorimetrically determined. Compared with the animals in the control group, it was determined that cattle with brucellosis had significantly (P<0.01) higher levels of AST, ALT, creatinine and NO and lower level of Fe. The increases of MDA and GSH levels were moderate and significantly important (P<0.05) while serum urea manner was not significantly altered. It was determined that significant alterations occurred in various biochemical parameters and antioxidant activity decreased in cattle with brucellosis.

This study aims to determine serum neopterin, procalcitonin, total sialic acid (TSA), paraoxonase-1 (PON1), and some haematological indices in calves with aspiration pneumonia (ASP). The patient group consisted of 30 calves of the Simmental breed, 0–28 days old, diagnosed with ASP upon clinical examination, anamnesis, auscultation examination, and radiographic results. The control group consisted of 10 healthy calves, otherwise with the same characteristics. A significant difference was found between calves with ASP and the control group of calves in serum iron (Fe, P < 0.001), total iron-binding capacity (TIBC, P < 0.05), PON1, TSA, procalcitonin, neopterin, and total leukocyte count (WBC) indices as a result of the analyses (P < 0.001). Serum procalcitonin concentration was found to be 285.71 ng/ml in the ASP group and 30.34 ng/ml in the control group. Serum neopterin concentration was found to be 37.68 nmol/l in the ASP group and 15.14 nmol/l in the control group. Serum procalcitonin, neopterin, and TSA concentrations were found to be significantly higher in the ASP group compared to the control group. It was concluded that biomarkers such as serum procalcitonin, neopterin, and TSA are high in calves with ASP, and as a result, these markers provide diagnostically and prognostically important information.