Monkeys serve as important model species for studying human diseases and developing therapeutic strategies, yet the application of monkeys in biomedical researches has been significantly hindered by the difficulties in producing animals genetically modified at the desired target sites. Here, we first applied the CRISPR/Cas9 system, a versatile tool for editing the genes of different organisms, to target monkey genomes. By coinjection of Cas9 mRNA and sgRNAs into one-cell-stage embryos, we successfully achieve precise gene targeting in cynomolgus monkeys. We also show that this system enables simultaneous disruption of two target genes (Ppar-γ and Rag1) in one step, and no off-target mutagenesis was detected by comprehensive analysis. Thus, coinjection of one-cell-stage embryos with Cas9 mRNA and sgRNAs is an efficient and reliable approach for gene-modified cynomolgus monkey generation.
The lattice structure is a type of cellular material with trusslike frames which can be optimized for specific loading conditions. The fabrication of its intricate architecture is restricted by traditional manufacturing technologies. However, additive manufacturing (AM) enables the fabrication of complex structures by aggregation of materials in a layer-by-layer fashion, which has unlocked the potential of lattice structures. In the last decade, lattice structures have received considerable research attention focusing on the design, simulation, and fabrication for AM techniques. And different modeling approaches have been proposed to predict the mechanical performance of lattice structures. This review introduces the aspects of modeling of lattice structures and the correlation between them, summarizes the existing modeling approaches for simulation, and discusses the strength and weakness in different simulation methods. This review also summarizes the characteristics of AM in manufacturing cellular materials and discusses their influence on the modeling of lattice structures.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.