As the prime criminal among all the cardio-vascular diseases, chronic heart failure (CHF) is still far from being fully understood after decades of study by researchers. The booming bioinformatics studies, especially the microRNA (miRNA) microarray analysis, have significantly accelerated the uncovering of underlying mechanisms of human diseases. However, these miRNA researches mainly focus on single miRNA, paying less attention to the group characteristics of miRNAs, which may ignore the group characteristics of miRNAs. Here we introduce a novel miRNA set concept incorporation with a group analysis method of CHF miRNA microarray expression. Our results show great accordance with previous studies, and also reveal potential characteristics of miRNAs in CHF. Furthermore, this novel miRNA set approach may give us new insights into other diseases studies as well.
A protocol is described for plant regeneration from protoplasts of Gentiana straminea Maxim. via somatic embryogenesis. Protoplasts were isolated from embryogenic calli in an enzyme solution composed of 2% Cellulase Onozuka R-10, 0.5% Macerozyme R-10, 0.5% Hemicellulase, and 0.5 M sorbitol with a yield of 3.0 × 106 protoplasts per gram of fresh weight. Liquid, solid-liquid double layer (sLD) and agar-pool (aPL) culture systems were used for protoplast culture. The aPL culture was the only method that produced embryogenic, regenerative calli. With aPL culture, the highest frequencies of protoplast cell division and colony formation were 39.6% and 16.9%, respectively, on MS medium supplemented with 2 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mg/L N6-benzylaminopurine (BA). Microcalli were transferred to solid MS medium containing a reduced concentration of 2,4-D (0.5 mg/L) to promote the formation of embryogenic calli. Somatic embryos developed into plantlets on MS medium supplemented with 2 mg/L BA at a rate of 43.7%.
The complete chloroplast genome of Tulipa buhseana was sequenced and reported here. The circular genome of T. buhseana is 152,062 bp in length and contains 133 functional genes consisting of 87 coding sequences, 38 tRNA genes, and 8 rRNA genes. With 1 species from Smilacaceae and 1 species from Alstroemeriaceae as outgroup, phylogenetic relationships of 8 Liliaceae species based on their chloroplast genomes indicated that T. buhseana is closest to T. altaica.
The chloroplast genome and evolutionary relationship analysis of Tulipa gesneriana L. could provide fundamental genetic reference for its molecular breeding and biological research. The complete chloroplast genome of Tulipa iliensis was sequenced and reported here. Its chloroplast genome was 151,744 bp in length, containing a pair of inverted repeated regions (26,354 bp) which were separated by a large single copy region of 81,794 bp, and a small single copy region of 17,242 bp. Moreover, a total of 133 functional genes were annotated, including 87 mRNA, 38 tRNA genes, and 8 rRNA genes.The phylogenetic relationships of 16 species indicated that T. iliensis was closely related to T. altaica.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.