BackgroundLimited evidence exists in the literature regarding whether a specific mount is preferable to use for processing endoscopically obtained gastrointestinal biopsy specimens.Hypothesis/ObjectivesTo compare 3 methods of handling endoscopically obtained gastrointestinal biopsy specimens from collection to laboratory processing and to determine if any technique produced superior results.AnimalsTwenty‐three dogs and cats presented for gastrointestinal signs.MethodsProspective study of dogs and cats presented with gastrointestinal signs to a veterinary teaching referral hospital which underwent upper gastrointestinal endoscopy. Biopsy specimens were taken from the stomach and duodenum and submitted to the laboratory using 3 techniques: mounted on a cucumber slice, mounted on a moisturized synthetic foam sponge, and floating free in formalin. The techniques were compared with regard to the specimens' width, orientation, presence of artifacts, and pathologist's confidence in diagnosis.ResultsTwenty‐three patients were included, with a total of 528 biopsies collected. Specimens on cucumber slice and on sponge were significantly wider (P < .001 and P = .001, respectively) compared to those floating free in formalin (mean width of 3.81 versus 3.31 and 2.52 mm, respectively). However, specimens on synthetic sponge had significantly fewer artifacts compared to those on cucumber slice (P = .05) and those floating free in formalin (P = .02). Confidence in the diagnosis also was superior with the sponge technique over floating free specimens (P = .002).Conclusions and Clinical ImportanceThe use of mounted gastrointestinal biopsy specimens was superior over the use of specimens floating free in formalin. This technique improved the quality of the specimens and the pathologist's confidence in their histopathologic interpretation.
A 10-year-old Jack Russell Terrier presented with a six-month history of chronic vomiting. Initial bloodwork and ultrasound demonstrated mild and non-specific changes. Vomiting dramatically improved upon feeding a home-made elimination diet, and clinical signs reappeared following a provocation trial. However, 12 weeks after the first visit, the dog became inappetent and lost weight despite this food change. A gastroscopy was performed, and histological examination of gastric mucosal samples revealed high-grade B-cell lymphoma, which was confirmed on immunohistochemistry. Systemic chemotherapy was initiated, and the dog survived 30 months on treatment. This is an exceptional long-term survival time of a dog presenting a gastric lymphoma and concurrent dietary intolerance.
A 14-week-old entire male springer spaniel was presented for haematuria. Investigations identified polypoid cystitis. Medical management failed to improve the clinical signs, and the dog underwent surgical excision of the bladder polyps at eight months old. Histopathological examination of the polyps identified granulomatous foci containing crystalline material. Eighteen months after surgery, the dog has had no further episode of haematuria and repeat ultrasound-documented resolution of the lesions. This is the youngest reported case of polypoid cystitis in dogs and the first report of this lesion associated with crystal inclusions within the polyps.
General rightsThis document is made available in accordance with publisher policies. Please cite only the published version using the reference above. Full terms of use are available: http://www.bristol.ac.uk/pure/about/ebr-terms Objectives: The aims of this study were to compare cytological samples obtained from endoscopic 6 biopsies using 'imprint' and 'squash' techniques, and to evaluate the potential value of cytology 7 compared to histology in reaching the diagnosis. 8Methods: Eighteen dogs and five cats undergoing endoscopy for chronic gastrointestinal signs were 9 prospectively included. Imprint and squash samples were obtained from one biopsy and then 10 analysed. Comparison between cytology and histology was performed using Cohen's kappa 11 coefficient.
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