The use of anaerobic biomass attached to a support has been recently presented as a good prospect in the treatment of wastewater containing recalcitrant compounds, such as sulfamethazine (SMZ). SMZ has been found in swine wastewater and sewage treatment plants, which motivates assessing their degradation by new wastewater treatment technologies. Thus, this paper describes the use of a continuous fixed structured bed bioreactor for the purpose of evaluating SMZ removal kinetics present in lab-made wastewater. The analysis of SMZ used online solid-phase extraction coupled to liquid chromatography/tandem mass spectrometry (SPE online-LC-MS/MS). Chemical oxygen demand (COD) was also monitored to evaluate the organic matter removal. The bioreactor was operated under mesophilic conditions (30[Formula: see text]C), with a hydraulic retention time of 24 h. In order to evaluate SMZ removal, four different concentration levels were studied: 200, 400, 600, and 800 ng L. COD removal efficiency obtained for filtered effluent kept at 91.01% and there was no interference due to the increase of SMZ concentration. For SMZ, the removal efficiencies were of [Formula: see text] for 200 ng L concentration level; [Formula: see text] for 400 ng L; [Formula: see text] for 600 ng L, and [Formula: see text] for 800 ng L. COD removal kinetics presented a first-order apparent removal rate constant ([Formula: see text]) of [Formula: see text] h. SMZ also showed a first-order apparent removal rate constant of [Formula: see text] h for the following concentrations levels: 200, 400, 600, and 800 ng L.
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