Site-specific
drug delivery is an effective approach to decreasing
drug toxicity and enhancing therapeutic effects. In this study, zein
was decorated with folic acid (FA) for targeted delivery of 10-hydroxycamptothecin
(HCPT). The reaction process was monitored using an online ATR-UV
spectrophotometer, and the conjugation degree was quantified using
a UV–vis spectrophotometer. Successful conjugation was evidenced
using FT-IR and 1H NMR. The influence of FA conjugation
on the self-assembly of zein molecules and cellular uptake was investigated
in detail. FA conjugation facilitates the formation of small nanoparticles
with good dispersity and stability and improves the cellular uptake
in folate receptor positive cells. HCPT nanocrystals (NC) were prepared
and incorporated into FA-zein. The drug release behavior of HCPT NC/FA-zein
nanoparticles is more consistent with a diffusion mechanism than a
matrix swelling/erosion mechanism of HCPT NC/zein particles; and HCPT
NC/FA-zein nanoparticles induce higher antitumor activity in folate
receptor positive cells at low HCPT-equivalent concentration. These
results suggest that FA-zein is a potential carrier material for sustained
and targeted delivery of anticancer drugs.
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