Abstract:In this study, a novel 96-well microplate swivel system (M96SS) was built for high-throughput screening of microalgal strains for CO 2 fixation. Cell growth under different CO 2 supply conditions (0.2, 0.4, 0.8, and 1.2 g L −1 d −1 ), residual nitrate, and pH value of Chlorella sp. SJTU-3, Chlorella pyrenoidosa SJTU-2, and Scenedesmus obliquus SJTU-3 were examined in the M96SS and traditional flask cultures. The dynamic data showed there was a good agreement between the systems. Two critical problems in miniature culture systems (intra-well mixing and evaporation loss) were improved by sealed vertical mixing of the M96SS. A sample screen of six microalgal species (Chlorella sp. SJTU-3, Chlorella pyrenoidosa SJTU-2, Selenastrum capricornutum, Scenedesmus obliquus SJTU-3, Chlamydomonas sajao, Dunaliella primolecta) was carried out in flasks and the M96SS. Chlamydomonas sajao appeared to be a robust performer (highest cell density: 1.437 g L −1 ) in anaerobic pond water with 0.8, and 1.2 g L −1 d −1 CO 2 .The reliability and efficiency of the M96SS were verified through a comparison of traditional flask culture, M96SS, Lukavský's system, and a microplate shaker.
Wastewater treatment technology with a simple processing unit, easy management, low energy consumption, low sludge production and a cost of 0.135 $ per m3.
Sequencing Batch Reactors (SBR) offers an effective way to achieve lower effluent limits for the treatment of complex and toxic coal gasification wastewater.
Coal gasification waste water treatment needed a sustainable and affordable plan to eliminate the organic contaminants in order to lower the potential environmental and human health risk. In this paper, a laboratory-scale anaerobic-aerobic intermittent system carried out 66 operational cycles together for the treatment of coal gasification waste water and the removal capacity of each organic pollutant. Contaminants included phenols, carboxylic acids, long-chain hydrocarbons, and heterocyclic compounds, wherein the relative content of phenol is up to 57.86%. The long-term removal of 77 organic contaminants was evaluated at different hydraulic retention time (anaerobic24 h + aerobic48 h and anaerobic48 h +aerobic48 h). Contaminant removal ranged from no measurable removal to near-complete removal with effluent concentrations below the detection limit. Contaminant removals followed one of four trends: steady-state removal throughout, increasing removal to steady state (acclimation), decreasing removal, and no removal. Organic degradation and transformation in the reaction were analysed by gas chromatography/mass spectrometry technology.
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