Boea hygrometrica resurrection plants require a period of acclimation by slow soil-drying in order to survive a subsequent period of rapid desiccation. The molecular basis of this observation was investigated by comparing gene expression profiles under different degrees of water deprivation. Transcripts were clustered according to the expression profiles in plants that were air-dried (rapid desiccation), soil-dried (gradual desiccation), rehydrated (acclimated) and air-dried after acclimation. Although phenotypically indistinguishable, it was shown by principal component analysis that the gene expression profiles in rehydrated, acclimated plants resemble those of desiccated plants more closely than those of hydrated acclimated plants. Enrichment analysis based on gene ontology was performed to deconvolute the processes that accompanied desiccation tolerance. Transcripts associated with autophagy and α-tocopherol accumulation were found to be activated in both air-dried, acclimated plants and soil-dried non-acclimated plants. Furthermore, transcripts associated with biosynthesis of ascorbic acid, cell wall catabolism, chaperone-assisted protein folding, respiration and macromolecule catabolism were activated and maintained during soil-drying and rehydration. Based on these findings, we hypothesize that activation of these processes leads to the establishment of an optimal physiological and cellular state that enables tolerance during rapid air-drying. Our study provides a novel insight into the transcriptional regulation of critical priming responses to enable survival following rapid dehydration in B. hygrometrica.
DNA methylation shapes the epigenetic landscape of the genome, plays critical roles in regulating gene expression, and ensures transposon silencing. As is evidenced by the numerous defects associated with aberrant DNA methylation landscapes, establishing proper tissue-specific methylation patterns is critical. Yet, how such differences arise remains a largely open question in both plants and animals. Here we demonstrate that CLASSY1-4 (CLSY1-4), four locus-specific regulators of DNA methylation, also control tissue-specific methylation patterns, with the most striking pattern observed in ovules where CLSY3 and CLSY4 control DNA methylation at loci with a highly conserved DNA motif. On a more global scale, we demonstrate that specific clsy mutants are sufficient to shift the epigenetic landscape between tissues. Together, these findings reveal substantial epigenetic diversity between tissues and assign these changes to specific CLSY proteins, elucidating how locus-specific targeting combined with tissue-specific expression enables the CLSYs to generate epigenetic diversity during plant development.
Geese form a fatty liver after feeding on a carbohydrate-rich diet, possibly as an evolutionary adaptation to accumulate reserves for migration. To gain insight into the gene-regulation processes of hepatic steatosis in geese, we examined the profile of transcriptional expression in goose fatty liver and control liver by suppression subtractive hybridization and measured the levels of serum biochemical variables. We found 107 genes whose expression was different between the treatment and control groups. The main functions of these genes are metabolic processes, including the metabolism of carbohydrates, amino acids, and lipids. Twenty-four genes were classified using the Kyoto Encyclopaedia of Genes and Genomes pathways. Twelve genes that related to metabolic and cellular processes were confirmed by quantitative RT-PCR. A specific positive effect of feeding was observed on the expression of genes involved mainly in unsaturated fatty acids and triglyceride synthesis, and a negative effect was observed on genes involved in β-oxidation, cholesterol metabolism, and glycolysis. The results could serve as an important reference for the development of goose breeding for fatty liver production and human liver disease research.
Vegetative tissues of Boea hygrometrica, a member of the Gesneriaceae family, can tolerate severe water loss to desiccated state and fully recover upon rehydration. Unlike many other so called “resurrection plants,” the detached leaves of B. hygrometrica also possess the same level of capacity for desiccation tolerance (DT) as that of whole plant. B. hygrometrica is distributed widely from the tropics to northern temperate regions in East Asia and grows vigorously in areas around limestone rocks, where dehydration occurs frequently, rapidly, and profoundly. The properties of detached B. hygrometrica leaves and relative ease of culture have made it a useful system to study the adaptive mechanisms of DT. Extensive studies have been conducted to identify the physiological, cellular, and molecular mechanisms underlying DT in the last decade, including specific responses to water stress, such as cell wall folding and pigment-protein complex stabilizing in desiccated leaves. In this review, the insight into the structural, physiological, and biochemical, and molecular alterations that accompany the acquisition of DT in B. hygrometrica is described. Finally a future perspective is proposed, with an emphasis on the emerging regulatory roles of retroelements and histone modifications in the acquisition of DT, and the need of establishment of genome sequence database and high throughput techniques to identify novel regulators for fully understanding of the matrix of DT.
Saurichthys dawaziensis sp. nov., from the third member of the Gejiu Formation (Anisian of Middle Triassic) in the vicinity of Luoping, Yunnan Province, is a small to medium‐ sized saurichthyid fish. It is characterized by the efferent pseudobranchial arteries penetrating on the parasphenoid and the mandible sensory canal with a branch in the angular. The new species is more derived than the Lower Triassic species in having a relatively narrow postorbital region, short preopercular, single ossification of the sclerotic ring, small haemal arches between the pelvic fins and the anal fin without ossified spines, and in the absence of quadratojugal. It is more primitive than the Middle and Upper Triassic Saurichthys in having fringing fulcra and numerous segments in the unpaired fins, and the presence of both prae‐ and post‐zygapophyses in the neural arches. Some anatomic changes related to the vertebral column and unpaired fins of the genus are also discussed.
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