MicroRNAs (miRNAs) are a group endogenous small non-coding RNAs that inhibit protein translation through binding to specific target mRNAs. Recent studies have demonstrated that miRNAs are implicated in the development of cancer. However, the role of miR-144 in uveal melanoma metastasis remains largely unknown. MiR-144 was downregulated in both uveal melanoma cells and tissues. Transfection of miR-144 mimic into uveal melanoma cells led to a decrease in cell growth and invasion. After identification of two putative miR-144 binding sites within the 3' UTR of the human c-Met mRNA, miR-144 was proved to inhibit the luciferase activity inMUM-2B cells with a luciferase reporter construct containing the binding sites. In addition, the expression of c-Met protein was inhibited by miR-144. Furthermore, c-Met-mediated cell proliferation and invasion were inhibited by restoration of miR-144 in uveal melanoma cells. In conclusion, miR-144 acts as a tumor suppressor in uveal melanoma, through inhibiting cell proliferation and migration. miR-144 might serve as a potential therapeutic target in uveal melanoma patients.
The solvothermal reactions via the self-assemble approach produced two new metal-organic compounds, i.e, [Zn(4-cptpy)(HCOO)(H2O)]n·n(DMF) (1) and [Cd3(btc)2(4-cptpyH)(DMF)(H2O)3]n (2) (DMF is N,N’-dimethylformamide, H3btc is benzene-1,3,5-tricarboxylic acid, and 4-Hcptpy is 4-[2,6-bis(pyridine-4-yl)pyridine-4-yl]benzoic acid). Furthermore, the compounds’ luminescent performances have also been studied. As a result, compounds 1-2 show intense luminescence at room temperature. The evaluation of their application values against postoperative infectious endophthalmitis (PIE) was implemented and their specific mechanism was investigated. First of all, the real time reverse transcription-polymerase chain reaction (RT-PCR) was performed and the nuclear factor kappa-B (NF-κb) signaling pathway activation levels were measured. In addition to this, the inflammatory cytokines content released after the cataract surgery was determined through exploiting the enzyme linked immunosorbent assay (ELISA) detection kit.
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