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Horehound herb (Marrubium vulgare L.) is harvested from plantations in Poland. In our country, there are also favorable conditions for M. incanum Desr. growing. The aim of the study was to compare the chemical composition of essential oils from M. vulgare L. and M. incanum Desr. in the second year of cultivation. The study revealed the presence of 31 compounds in the essential oil from M. vulgare L. and 24 compounds in the essential oil from M. incanum Desr. Chromatographic analysis revealed that the main compounds in the essential oil of M. vulgare L. from a two-year plantation were as follows: E-caryophyllene (34.51-36.78%), germacrene D (22.45-27.18%), bicyclogermacrene (9.54-11.12%), δ-amorphene (6.15-8.18%), and carvacrol (4.71-6.64%), whereas the following compounds prevailed in the oil from M. incanum Desr.: germacrene D (28.75-32.14%), E-caryophyllene (23.18-29.57%), α-cadinol (13.59-20.84%), and carvacrol (2.08-7.47%).

S u m m a r yThe study upon sage (Salvia officinalis L.) has been carried out at the Experimental Section of Department of Vegetables and Medicinal Plants, University of Life Sciences in Lublin. The aim of present study was to evaluate the yield and chemical composition of essential oil extracted from sage leaves harvested from a three-year plantation. The sage herb harvest date in the third year of cultivation had a significant impact on the yield of plants. Higher yield of fresh and dry herb, dry leaves, and essential oil was achieved in August (the second harvest time) than in May (the first harvest time). Chromatographic examination indicated the presence of 50 chemical compounds in sage essential oil. It was a variable percentage of essential oil components depending on the raw material harvest time. The main components of the sage essential oil were: 1,8-cineole (16.08-18.04%), α-thujone (10.40-21.51%) and camphor (5.24-18.08%).

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The chemical composition and activity of herbal raw material depend, among others, on ontogenetic variability. The present study investigated the effect of harvest date of winter savory (Satureja montana L.) herb and plant age on fresh and dry herb yield as well as on the content of L-ascorbic acid, carotenoids, chlorophylls, essential oil, flavonoids and tannins. S. montana herb from a one-and two-year-old plantation was harvested in June (vegetative stage), July (beginning of flowering), August (full flowering) and September (senescent plants). As the plants progressed to the successive growth stages, they were characterized by a higher fresh and dry herb yield. Two-year-old plants produced a significantly higher fresh and dry herb yield compared to one-year-old plants. The herb harvested before flowering contained most L-ascorbic acid, carotenoids and tannins, whereas the herb harvested at full floweringmost chlorophylls, essential oil and flavonoids. Two-year-old plants accumulated in the herb more L-ascorbic acid, chlorophylls and essential oil than one-year-old plants.