Immunoblotting of the separated membrane components of MiI erythrocytes with anti-Vw identified a band of M(r) 40,000 with a mobility close to that of β-sialoglycoprotein corresponding to the abnormal α-sialoglycoprotein present in Mil cells. A comparison of results obtained when MiIII erythrocytes were immunoblotted with anti-Mur, anti-s and the monoclonal antibody R1.3, indicated that the Mur antigen is located on the abnormal δ-sialoglycoprotein of Mr 36,000 in MiIII erythrocytes. Prior treatment of MiI erythrocytes with neuraminidase resulted in an increase in the intensity of staining of the anti-Vw reactive component. This was consistent with the enhanced reactions observed in haemagglutination tests with neuraminidase-treated erythrocytes. The mobility of the Vw component was reduced when erythrocytes were pre-treated with low concentrations of neuraminidase but increased when higher concentrations of neuraminidase were used.
This paper engages in a cross-cultural discourse analysis of two reality television programs: the Japanese program Terrace House and the American program The Real World. The main goal of the analysis was to understand the conflict styles of the participants in each program, and explore the differences and/or similarities between them. Generally, the programs differed significantly in their portrayals of conflict with the Japanese program demonstrating that participants tended to attempt to maintain harmony among the group, and the American program demonstrating that participants focused more on individual concerns in conflict situations. Terrace House emphasized the resolution and management of conflict. The Real World emphasized the escalation of conflict and its management only when it became extreme. This work did identify a limited selection of similarities between the conflict management styles of both groups, however.
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