Identification by Immunoblotting of the Erythrocyte Membrane Sialoglycoproteins That Carry the Vw and Mur Antigens

Abstract

Immunoblotting of the separated membrane components of MiI erythrocytes with anti-Vw identified a band of M(r) 40,000 with a mobility close to that of β-sialoglycoprotein corresponding to the abnormal α-sialoglycoprotein present in Mil cells. A comparison of results obtained when MiIII erythrocytes were immunoblotted with anti-Mur, anti-s and the monoclonal antibody R1.3, indicated that the Mur antigen is located on the abnormal δ-sialoglycoprotein of Mr 36,000 in MiIII erythrocytes. Prior treatment of MiI erythrocytes with neuraminidase resulted in an increase in the intensity of staining of the anti-Vw reactive component. This was consistent with the enhanced reactions observed in haemagglutination tests with neuraminidase-treated erythrocytes. The mobility of the Vw component was reduced when erythrocytes were pre-treated with low concentrations of neuraminidase but increased when higher concentrations of neuraminidase were used.

Miltenberger Subsystem of the MNSs Blood Group System: Review and Outlook

“…31-49 of the Mi.111 and Mi.VI variants was found to inhibit 'anti-Vw+Hut+Mur'. Recently, other authors [22] have reported that 'anti-Mur' binds to Mi .HI-GPB-A-B. However, the author concludes that other related antibody specificities (anti-Mia, anti-Vw plus anti-Hut+Mur) have not been excluded.…”

“…Inhibition experiments demonstrated that the Mi' and Vw antigens are located in the region of G P A exhibiting the Mi.1-or Mi.11-specific alterations [21]. Recently, the location of the Vw antigen on Mi.1-GPA has been confirmed by immunoblotting [22]. A recent abstract describes the first Mi.…”