Flood events typically enhance primary productivity in estuaries via the increased nutrient inputs from land runoff. This study examined the drivers of phytoplankton biomass accumulation and productivity in a tropical estuary with a distinct wet-dry seasonality, i.e. months of little or no rainfall, and a highly episodic extended wet season. The study found that over two wet seasons, there was little evidence of freshwater inputs increasing nutrient concentrations, and chlorophyll a concentrations and phytoplankton productivity rates decreased in the water column, probably due to low water residence times. The magnitude and duration of freshwater flows in the wet season appeared to affect the scale of reduction of phytoplankton productivity and biomass accumulation. In contrast to many studies, there was also no evidence of post-flooding stimulation of chlorophyll a concentration with net export of nutrients in both the wet and dry seasons. Nitrogen (N) and light appeared to be key limiting factors for phytoplankton growth with estuarine DIN rapidly turned over by phytoplankton, no evidence of N fixation by phytoplankton, and a response to N, but not phosphorus (P), in algal bioassays. Tidal resuspension of sediments was an important physical process that limited light availability for primary productivity. The lack of higher nutrient concentrations as a result of freshwater inputs, and lack of post-flood algal growth stimulation contrasts with the findings of studies in eutrophied systems. in turn, fuel primary production in the months during and post-flooding once flow and turbidity have decreased, and salinity has increased (Mallin et al., 1993; Gillanders and Kingsford, 2002; Murrell et al., 2007). Wet and dry tropical/subtropical river systems account for 68% of Australian estuaries, meaning that they are an important contributor to the productivity of estuarine and coastal systems (Bucher and Saenger, 1994). Indeed studies have shown a correlation between catches of estuarine fish and crustaceans, and measures of freshwater flow which suggests that freshwater inputs may be providing nutrients and carbon to fuel productivity in estuaries (Gillanders and Kingsford, 2002; Robins et al., 2005). Conversely, estuarine productivity appears to be negatively affected if the upstream river is regulated, reducing the magnitude of high flow events (Kenyon et al., 2004; Burford et al., 2011). Eyre (2000) has proposed that estuaries of the wet and dry tropics/subtropics have four regime states throughout a typical year. During floods, freshwater flushes nutrients and sediments out to sea. This is followed by a recovery phase where turbidity begins to decrease, however low light availability prevents phytoplankton growth. In the medium flow phase, increasing light and nutrient availability stimulates phytoplankton biomass. Finally, in the dry season, phytoplankton become nutrient limited again, with a resulting decrease in biomass. This study therefore examined the role of freshwater nutrient inputs in stimulating phytopl...