Background Mexico is considered the diversification center for chili species, but these crops are susceptible to infection by pathogens such as Colletotrichum spp., which causes anthracnose disease and postharvest decay in general. Studies have been carried out with isolated strains of Colletotrichum in Capsicum plants; however, under growing conditions, microorganisms generally interact with others, resulting in an increase or decrease of their ability to infect the roots of C. chinense seedlings and thus, cause disease. Results Morphological changes were evident 24 h after inoculation (hai) with the microbial consortium, which consisted primarily of C. ignotum. High levels of diacylglycerol pyrophosphate (DGPP) and phosphatidic acid (PA) were found around 6 hai. These metabolic changes could be correlated with high transcription levels of diacylglycerol-kinase (CchDGK1 and CchDG31) at 3, 6 and 12 hai and also to pathogen gene markers, such as CchPR1 and CchPR5. Conclusions Our data constitute the first evidence for the phospholipids signalling events, specifically DGPP and PA participation in the phospholipase C/DGK (PI-PLC/DGK) pathway, in the response of Capsicum to the consortium, offering new insights on chilis’ defense responses to damping-off diseases.
Mexico is considered to be a diversification center for the chili species, but at the same time, these crops have been susceptible to infection by pathogens, such as Colletotrichum, which causes anthracnose diseases and postharvest decay in general. Different studies have been carried out with isolated strains of Colletotrichum in Capsicum plants, however, under growing conditions, the microorganisms are interacting with others, which can increase or decrease their infective capacity. This study presents the first report between plant-pathogen interactions and their biochemical responses in phospholipid pathways for C. chinense to microbial consortium with mainly Colletotrichum ignotum pathogen. The results showed morphological changes in the first hours (h) in the presence of the microbial consortium, and high levels of diacylglycerol pyrophosphate (DGPP) and phosphatidic acid (PA) were found after 6 h postinoculation (hai). These metabolic changes were correlated with high transcription levels of diacylglycerol-kinase (CchDGKs) expressed for 3, 6 and 12 hai and related to pathogen gene markers, such as CchPR1 and CchPR5. Finally, this study shows how the phospholipase C/DGK (PLC/DGK) pathway offers insight into the microbial infection responses of chili crops with damping-off diseases in the Yucatan.
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