Medicinal plants play a key role in human health care. Frustration over the side effects of allopathic drugs has driven the medical world to take asylum in the plant kingdom for the treatment of various ailments. Euphorbia hirta belonging to the family of Euphorbiacae has been reported to possess antibacterial, antiviral, and anticancer activity. The aim of the present study was to investigate the protective effect of E. hirta against antitubercular druginduced cytotoxicity in freshly isolated hepatocytes. The extent of cytotoxicity of the plant extracts was also analyzed using human liver derived HepG2 cell line by estimating the viability of cells (MTT assay). The alcoholic plant extract normalized the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), triacylglycerol (TAG), cholesterol, total protein, albumin, total and direct bilirubin, which were altered due to antitubercular drug intoxication. A dose-dependent increase in percent viability was observed when antitubercular drug exposed HepG2 cells were treated with different concentrations of plant extracts (125, 250, 500 and 1000 μg/mL) which were compared with a standard hepatoprotective drug Silymarin. The highest percentage viability of HepG2 was observed at a concentration of 1000 μg/mL. The results suggest that E. hirta exerts protection against antitubercular drug-induced cytotoxicity in this vitro model system.
Ginning factories discharge large amounts of cotton dusts that lead to decreased pulmonary function in the exposed subjects. An attempt was made to study the biochemical profile of women laborers employed in ginning factory located in Tirupur, a textile based city in Coimbatore district of Tamilnadu, India. The blood parameters that were analyzed were hemoglobin, total and differential count of leucocytes, erythrocyte sedimentation rate (ESR), total proteins, immunoglobulins, total and isozymic content of lactate dehydrogenase (LDH) and histamine. Student's ‘t’ test was carried out to compare the results with the control women. Correlation analysis was done between/within the biochemical parameters and also between the pulmonary function parameters results reported earlier by Jannet and Jeyanthi. Significant changes in the levels of hemoglobin, ESR, immunoglobins and histamine were reported in this study. Correlation studies between the pulmonary function parameters and biochemical parameters revealed significant negative correlation of FVC, FEV1 and PEF with ESR (P <0.05). There was also positive correlation between immunoglobin G and histamine. A significant negative correlation was observed between LDH1 and LDH3 and between albumin and γ globulin. The study suggested that the ginning factory women laborers exhibited significant changes in the levels of certain biochemical parameters apart from the pulmonary functional changes.
Purpose:The present study was designed to study the alphaamylase inhibitory property and antioxidant potential of the various solvent extracts of the bark of two medicinal plants Saraca indica and Polyalthia longifolia.Method:The yield percentage of aqueous, ethanol, ethyl acetate, hexane and chloroform extracts of Saraca indica and Polyalthia longifolia bark were assessed by extracting 5 g of the dried plant material in 25 ml of the respective solvent in a shaker at 40 degree celsius for 48 hours. Filtered and evaporated the solvents at room temperature. Aqueous extract was subjected to lyophilization. The residues were weighed to calculate the yield. Total antioxidant potential of the extracts were assessed by FRAP assay and compared with standard ascorbic acid. Inhibition of in vitro lipid peroxidation of the potent extract was studied using linolenic acid micelles as model system. Alphaamylase inhibitory property of the extracts was studied by the method given by Bernfield (1995) and compared with that of the standard inhibitor acarbose. Also the type of inhibition of the potent extracts was studied by Dixon plot.Result:The results revealed that the yield percentage was highest for ethanolic extracts of Saraca indica and Polyalthia longifolia bark. Among all the tested extracts the ethanolic extract of both the barks found to possess highest antioxidant potential and alpha amylase inhibitory activity followed by the aqueous and ethylacetate extracts. Hexane and chloroform extracts possessed lowest antioxidant and amylase inhibition potential. The ethanolic extracts presented good inhibition of in vitro lipid peroxidation. The type of inhibition of amylase showed by the ethanolic extracts was found to be competitive.Conclusion:To conclude, the ethanolic extracts of Saraca indica and Polyalthia longifolia bark were found to possess good antioxidant potential and also showed efficient inhibition of the enzyme alphaamylase.
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