An antimicrobial validatory screening of short nosed tripod fish Triacanthus biaculeatus (Bloch, 1786) collected from the Visakhapatnam coastal waters, India is done in such a way T. biaculeatus muscle extracts were subjected for antimicrobial assay. Antimicrobial screening assay was done in five bacterial pathogens viz., Escherichia coli (MTCC-443), Salmonella typhi (MTCC-421), Pseudomonas aeruginosa (MTCC-2295), Vibrio cholera (MTCC-459) and Staphylococcus aureus (MTCC-3160) using the standard well diffusion method. The results confirmed a positive test against most of the pathogens used. Maximum antimicrobial effect against Vibrio cholera of 3.8mm in diameter is reported. The present investigation has revealed that positive progresses in the fish (T. biaculeatus) muscle extracts against human pathogens.
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