Experiments have been conducted to characterize the growth of Spodoptera frugiperda (fall armyworm) (Sf9) cells in standard and modified spinner designs. Results suggest that in standard spinner flasks growth is limited by mixing and oxygen transfer. Modifications to spinner geometry, baffling and agitator aspect ratio provided increases in growth rate and final cell density. Improvements in the performance of spinners, such as those described herein, have the potential to simplify a process and decrease the chance of contamination by decreasing the number of required manipulations and increase process productivity by decreasing inoculum train cycle times.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.