Background. The aim of this study was to investigate myofibroblast (MF) density in a broad spectrum of odontogenic cysts and tumors and the relation between the density of MFs and the clinical behavior of these lesions.Methods. A total of 105 cases of odontogenic lesions, including unicystic ameloblastoma (UAM), solid ameloblastoma (SA), odontogenic keratocyst (OKC), dentigerous cyst (DC), radicular cyst (RC) (15 for each category), and odontogenic myxoma (OM), adenomatoid odontogenic tumor (AOT), calcifying odontogenic cyst (COC) (10 for each category), were immunohistochemically stained with anti-α-smooth muscle actin antibody. The mean percentage of positive cells in 10 high-power fields was considered as MF density for each case.Results. A statistically significant difference was observed in the mean scores between the study groups (P < 0.001). The intensity of MFs was significantly higher in odontogenic tumors compared to odontogenic cysts (P < 0.001). There was no statistically significant difference between odontogenic tumors, except between UAM and OM (P = 0.041). The difference between OKC and odontogenic tumors was not statistically significant (P > 0.05). The number of MFs was significantly higher in OKC and lower in COC compared to other odontogenic cysts (P = 0.007 and P = 0.045, respectively).Conclusion. The results of the present study suggest a role for MFs in the aggressive behavior of odontogenic lesions. MFs may represent an important target of therapy, especially for aggressive odontogenic lesions. Our findings support the classification of OKC in the category of odontogenic tumors.
To examine the effect of a variety of irrigation systems on the removal of root canal Ca (OH)2 residues through meta-analysis and systematic review. Material and Methods: A search of relevant articles was systematically performed on databases of Medline, PubMed, Cochrane Library, Embase, ISI, and Google Scholar published from 2013 to 2019. Electronic title management was carried out by EndNote X9 software. Searches were based on the main keywords of "Irrigation Systems", "Calcium Hydroxide"," Root Canal", "Self-adjusting File OR SAF", "Conventional Irrigation", "EndoVac System", "Ultrasonic Irrigation" and "YAG Laser". Results: The studies investigated were found to lack any standardization concerning the irrigation method used or the measurement of outcomes; for instance, different studies used 5 or 10 mL of 2.5% NaOCl, or 5 mL of 5.25% NaOCl, or 0.5 mL, 3 mL and 10 mL of 17% EDTA. Conclusion: The effective techniques for the removal of root canal Ca(OH)2 were PUI, and SAF approaches.
To analyze data obtained from animal and human studies using stem cells. Material and Methods: Medical Literature Analysis and Retrieval System Online (MEDLINE), PubMed, Cochrane Library, Embase, Information Sciences Institute (ISI), as well as Google Scholar were utilized and searched as available electronic databases to perform a systematic literature review of articles published between 2010 and 2019. The Endnote X9 for Windows was also employed to manage electronic titles and abstracts of the selected studies. Searches were conducted using keywords of "pulpal OR pulpal tissue OR pulpal vitality", "regeneration", "apical healing", "stem cells OR progenitor cells", and "mediated pulpal tissue". Consequently, 189 titles and abstracts endowed with potential relevance were discovered based on searches into manual and electronic sources. Ultimately, a total of six articles met the inclusion criteria in the present systematic review and meta-analysis. Results: Out of the six articles identified and selected, five studies were categorized as animal experiments and one article was nominated as a human clinical trial. The greatest bias risks were accordingly observed in the majority of animal examinations, but articles related to humans revealed decreased risks of bias, while the human clinical trial showed some concerns. Conclusion: Promising parameters testing functional pulp regeneration could be represented through stem cell transplants.
BackgroundThe aim of this in vitrostudy was to examine and compare the effect of photodynamic therapy and solution containing nano particles Ag/ZnO on adhesion of endodontic sealers to dentinal walls of human root canal.Material and MethodsNinty single-rooted human teeth were selected and their clinical crown was cut from the cemento-enamel junction zone. Canals were prepared by RaCe rotary system and the smear layer was removed using 17% EDTA and 5.25% NaOCl. Samples were randomly divided into two groups of AH Plus and MTA Fillapex based on the sealer type and each group based on antimicrobial method was divided into two sub-groups of photodynamic therapy and polymer containing nano particles of Ag / ZnO and a control sub-group (N = 15). After obturation of canals with gutta-percha and sealers mentioned, the samples were incubated for a week at a humidity of 95% and 37°C and then 2mm thick discs were prepared from the middle region of roots for Push-out test. The maximum failure force was recorded in newton and converted to MPa. Then, 3 random specimens of each subgroup were evaluated by scanning electron microscopy. Statistical analysis was performed by Two way ANOVA and (P<0.05) was considered significant.ResultsThe highest (4.84 ± 1.63) and lowest (0.16 ± 0.075) push-out bond strength values were obtained in (AH Plus-PDT) and (MTA Fillapex-Ag/ZnO) respectively. Independent of antimicrobial method, AH Plus bond strength was significantly higher than the MTA Fillapex (p<0.001). For both sealers, PDT increased the bond strength significantly (p<0.001).ConclusionsPhotodynamic therapy has a positive effect on the bond strength of AH Plus and MTA Fillapex sealers. Key words:AH Plus, MTA Fillapex, Nano particles, Photodynamic therapy, Push-out.
Background: The effect of different intracanal medicaments on root fracture resistance has not been thoroughly investigated in the short and long term. To assess the effect of calcium hydroxide (CH), CH combined with Chlorhexidine (CHX), double antibiotic paste (DAP), and simvastatin as intracanal medicaments on the fracture resistance of the human root. One hundred and twenty single-rooted mandibular premolars which were extracted for periodontal reasons were collected for this in vitro study. Materials and Methods: This was an in vitro study. All teeth were decoronated. Root canals were prepared by the Pro taper system, and %2.5 NaOCl was used for irrigation. The smear layer was removed using %5.25 NaOCl and 17% ethylenediaminetetraacetic acid each for 3 min. The samples were randomly divided into five groups based on the medicament: (1) CH (2) CH + CHX (3) Simvastatin (4) DAP (5) Control group. All specimens in each group were incubated for 1 week (Subgroup A) and 1 month (Subgroup B). Then, medicaments were removed and filled with gutta-percha and AH26 sealer. All samples were tested for fracture resistance. The data were statistically evaluated with the SPSS software 17. ANOVA and Mann–Whitney U and Wilcoxon tests were used for the analysis of the data. P = 0.05 was considered statistically significant. Results: Although CH and CH + CHX increased the fracture resistance in a 1-week period, there was no significant difference between the groups after 1 month. Conclusion: Under the limitations of this study, CH and CH + CHX, DAP and simvastatin do not have a negative effect on root fracture resistance when used as intracanal medicaments for <1 month.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.