To examine the effect of a variety of irrigation systems on the removal of root canal Ca (OH)2 residues through meta-analysis and systematic review. Material and Methods: A search of relevant articles was systematically performed on databases of Medline, PubMed, Cochrane Library, Embase, ISI, and Google Scholar published from 2013 to 2019. Electronic title management was carried out by EndNote X9 software. Searches were based on the main keywords of "Irrigation Systems", "Calcium Hydroxide"," Root Canal", "Self-adjusting File OR SAF", "Conventional Irrigation", "EndoVac System", "Ultrasonic Irrigation" and "YAG Laser". Results: The studies investigated were found to lack any standardization concerning the irrigation method used or the measurement of outcomes; for instance, different studies used 5 or 10 mL of 2.5% NaOCl, or 5 mL of 5.25% NaOCl, or 0.5 mL, 3 mL and 10 mL of 17% EDTA. Conclusion: The effective techniques for the removal of root canal Ca(OH)2 were PUI, and SAF approaches.

To analyze data obtained from animal and human studies using stem cells. Material and Methods: Medical Literature Analysis and Retrieval System Online (MEDLINE), PubMed, Cochrane Library, Embase, Information Sciences Institute (ISI), as well as Google Scholar were utilized and searched as available electronic databases to perform a systematic literature review of articles published between 2010 and 2019. The Endnote X9 for Windows was also employed to manage electronic titles and abstracts of the selected studies. Searches were conducted using keywords of "pulpal OR pulpal tissue OR pulpal vitality", "regeneration", "apical healing", "stem cells OR progenitor cells", and "mediated pulpal tissue". Consequently, 189 titles and abstracts endowed with potential relevance were discovered based on searches into manual and electronic sources. Ultimately, a total of six articles met the inclusion criteria in the present systematic review and meta-analysis. Results: Out of the six articles identified and selected, five studies were categorized as animal experiments and one article was nominated as a human clinical trial. The greatest bias risks were accordingly observed in the majority of animal examinations, but articles related to humans revealed decreased risks of bias, while the human clinical trial showed some concerns. Conclusion: Promising parameters testing functional pulp regeneration could be represented through stem cell transplants.

Zn-doped CuFe2O4 nanoparticles (NPs) were eco-friendly synthesized using plant extract. These nanoparticles were characterized by X-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy and thermal gravimetric analysis (TGA). SEM image showed spherical NPs with size range less than 30 nm. In the EDS diagram, the elements of zinc, copper, iron, and oxygen are shown. The cytotoxicity and anticancer properties of Zn-doped CuFe2O4 NPs were evaluated on macrophage normal cells and A549 lung cancer cells. The cytotoxic effects of Zn-doped CuFe2O4 and CuFe2O4 NPs on A549 cancer cell lines were analyzed. The Zn-doped CuFe2O4 and CuFe2O4 NPs demonstrated IC50 values 95.8 and 278.4 µg/mL on A549 cancer cell, respectively. Additionally, Zn-doped CuFe2O4 and CuFe2O4 NPs had IC80 values of 8.31 and 16.1 µg/mL on A549 cancer cell, respectively. Notably, doping Zn on CuFe2O4 NPs displayed better cytotoxic effects on A549 cancer cells compared with the CuFe2O4 NPs alone. Also spinel nanocrystals of Zn-doped CuFe2O4 (~ 13 nm) had a minimum toxicity (CC50 = 136.6 µg/mL) on macrophages J774 Cell Line.

Background: Lack of knowledge of root anatomy is the second most common reason for failed endodontic therapy. Therefore, acquiring knowledge about it leads to a more effective treatment. C-shaped canal is an anatomical variation that causes clinical challenges. The objective of this study is to determine the occurrence of the C-shaped roots in permanent anterior and posterior teeth of the Iranian population using CBCT (cone beam computed tomography) scans. Methods: This was a descriptive cross-sectional study. In this study, 1408 teeth were assessed. Cranex 3D Mid was used to prepare CBCT images. Each tooth was examined in axial sections, and five different levels were identified as coronal, medium, apical, 1/3, and 2/3. The canal shapes were classified into 5 categories based on modified Melton’s method. Data were analysed using the chi-square test at a significance level of 5%in SPSS version 24.0. Results: In total, of 1408 teeth which were evaluated, 7.81% (110) were confirmed to have C-shaped canals. The occurrence of the C-shaped canal was significantly higher in molars (P < 0.001). The occurrence of the C-shaped canal was significantly higher in mandibular teeth (10.04%) compared to maxillary teeth (5.81%) (P < 0.001). The most common C-shaped canal category was C1 (57.27%). The relationship of gender and age with the C-shaped canal occurrence was not statistically significant (P = 0.585, P = 0.562, respectively). Conclusions: CBCT is a useful tool to evaluate C-shaped root canal morphology. The high occurrence of the C-shaped canal in the Iranian population requires clinical awareness.

BackgroundThe main reason for the failure of endodontic therapy is the incomplete knowledge about the anatomical variation of root canals. One of the most important factors that leads to the failure of root canal treatment, is missed and untreated major root canals.Objectivewith respect to the complexity of mandibular incisors treatment and high prevalence of the second canal, and the possibility of the relationship between the crown size and the extra canal in these teeth, the aim of this study was to determine the relationship between crown size and root canal morphology in mandibular incisors with CBCT.MethodsIn this cross-sectional study, mandibular permanent incisors were randomly collected in Qazvin City, Iran, and were mounted in eight ternary groups on a plastic slot, using putty molding material. After preparation of Scot view, the samples were scanned by CBCT NewTom 5G. Afterward, the mesiodistal and buccolingual dimensions were measured by the software’s measurement tool with a precision within tenths of a millimeter. In the next stage, a multi-planar option and 400% magnification tool of the software were utilized to study axial and cross sectional views of each tooth to determine canal type. Data were analyzed employing one-sample Kolmogorov-Smirnov, Levene, independent- samples t-test and Roc curve by SPSS version 20.ResultsThe majority of mandibular incisors have a single canal (63.9% of them had type I canal system). In addition, 36.1% of the roots had two canals, among which, type III was the most common. The mean of maximum mesiodistal and buccolingual diameters in type III was significantly bigger than that in type I (p<0.05), but the means of crown size in the two canal types were not significantly different.ConclusionDespite increase in mesiodistal and buccolingual dimension in two canal mandibular incisors with type III canal system, their crown sizes (M-D/F-L index) were not significantly different, in comparison to single canal incisors.

The vitality pulp tests are necessary for diagnosis of pulpal disease and differentiation between endodontic and nonendodontic disease. Thermal and electrical tests are conventional method for evaluation pulp vitality. The aim of the present study was to evaluate the accuracy of thermal and electrical tests to register pulp vitality in different group of teeth. 184 teeth (81 male, 103 female) with unknown pulpal status that need endodontic treatment were examined. After complete isolation, the thermal and electrical tests were performed. The cold, heat and electrical tests were done. The interval between tests was conducted after 2 minutes. Based on information obtained the sensitivity, specifi city, accuracy, positive and negative predictive value were calculated. In total, 78 teeth with necrotic pulp and 106 teeth with vital pulp were tested. The gold standard was established by direct pulp inspection of 184 teeth with endodontic treatment. Based on information obtained the accuracy of cold test in anterior teeth was 78/8%, in premolar teeth was 81.8% and in molar teeth was 80.5%. The accuracy of heat test in anterior teeth was 82.6%, in premolar teeth was 78.1% and in molar teeth was 72.7%. The accuracy of electric pulp test in anterior teeth was 71.1%, in premolar teeth was 74.5% and in molar teeth was71.4%. In anterior teeth the heat test has more accuracy than the other one. In premolar and molar teeth the cold test has more accuracy than other test.

Mesenchymal stem/stromal cells (MSCs)‐based therapy brings the reassuring capability to regenerative medicine through their self‐renewal and multilineage potency. Also, they secret a diversity of mediators, which are complicated in moderation of deregulated immune responses, and yielding angiogenesis in vivo. Nonetheless, MSCs may lose biological performance after procurement and prolonged expansion in vitro. Also, following transplantation and migration to target tissue, they encounter a harsh milieu accompanied by death signals because of the lack of proper tensegrity structure between the cells and matrix. Accordingly, pre-conditioning of MSCs is strongly suggested to upgrade their performances in vivo, leading to more favored transplantation efficacy in regenerative medicine. Indeed, MSCs ex vivo pre-conditioning by hypoxia, inflammatory stimulus, or other factors/conditions may stimulate their survival, proliferation, migration, exosome secretion, and pro-angiogenic and anti-inflammatory characteristics in vivo. In this review, we deliver an overview of the pre-conditioning methods that are considered a strategy for improving the therapeutic efficacy of MSCs in organ failures, in particular, renal, heart, lung, and liver.

Introduction:The exact measurement of the root canal length is of great importance in root canal therapy. Although determination of the root canal length through radiography is the most common method, recently electronic apex locators have been used to determine working length and decrease the number of radiographs. The purpose of this study was to compare the accuracy of an electronic apex locator (Root ZX) with conventional radiography to determine the working length (WL) of curved mandibular molars. Materials and methods:Thirty-five intact mandibular first molars with curve above 10 0 were selected. The access cavity was prepared and the root canal length was measured using apex locator and conventional radiography. Data were analyzed using the paired-sample t-test. Results:The working lengths measured by the apex locator were equal to the actual working lengths in 42.86%, 0.5-1 mm shorter in 31.43%, and 0.5 mm longer in 25.71%. The working lengths measured by radiography were equal to the actual working length in 51.14%, 0.5-1 mm shorter in 39.99%, and 0.5 mm longer in 2.86%. There was no significant difference between these two methods in the measurement of the working length (p = 0.951). Conclusion:The apex locator and conventional radiography were equally effective in determining the working length of the curved canals.