Membrane lipid peroxidation and DNA, protein damage is mediated by free radicals, which form the basis of chronic pathological complications.AgNPs are an important class of nanomaterials for a wide range of biomedical applications. The present study endeavors in vitro antioxidant and anti-inflammatory activity of green synthesized silver nanoparticles (AgNPs) using medicinal plant extract from Caesalpinia bonducella seeds. Total flavonoid and phenolic contents were determined. The antioxidant potential of capped AgNPs was assessed using DPPH assay, Phosphomolybdenum assay, FRAP assay, metal chelating, hydrogen peroxide, and hydroxyl radical scavenging methods. In vitro anti-inflammatory assay of CB seed, AgNPs were performed against the standard drug. CB seed AgNPs possessed high flavonoid and phenol compared to aqueous CB seed extract. The antioxidant methods confirmed that the silver nanoparticles have more antioxidant activity as compared to vitamin C. The synthesized silver nanoparticles exhibited potential anti-inflammatory activity with the IC50 71.3µg/ml. Hence, this work clearly demonstrated that the coated AgNPs with CB seeds act as a potent free radical scavenger and could be considered as a potential source for anti-inflammatory drugs.
Drug resistance and poor therapeutic outcomes are the emerging problems pertaining to cisplatin treatment in ovarian cancer. The effectiveness of the conventional chemotherapeutic medication could be improved by combining with natural drugs. In the current study, Wedelolactone (WDL) a natural coumestan, in combination with Cisplatin (Cis) was determined to be a potent anti-cancer drug as evidenced by their capacity to bring about cytotoxicity by decreasing NF-κB expression in PA-1 ovarian cancer cells. “Cell viability assays” were carried out and the effective combination of wedelolactone with Cisplatin were confirmed by PCR and western blot analysis. The determined IC50 (10µM) of WDL displayed advantageous anti-cancer effect in PA-1 cells compared to Cis treatment. Furthermore, the combination of wedelolactone (5µM) and cisplatin(3µM) also down regulated NF-κB expression which is a key player of various cancer promoting events such as drug resistance, apoptotic inhibition, inflammation and angiogenesis. WDL potentiates the sensitivity of PA-1 cells towards cisplatin by decreasing the ETS1 and P-gp expression which are involved in MDR mechanism. Overall, this study suggest that Wedelolactone can be used to sensitize ovarian tumors to standard cancer chemotherapeutics.
Naringin is a citrus flavonoid recently studied for anti-inflammatory activity in numerous cancer cells. In this study, the anti-inflammatory properties of naringin along with 5-fluorouracil in human skin cancer cell lines A375 was analyzed. A375 cells were treated with naringin, 5-fluorouracil alone, and combination. MTT assay and cell viability assays was demonstrated to detect the inhibitory effects of naringin or 5-fluorouracil on cell proliferation. mRNA expression of TNFα, IL-6, IL-1β, and NFκB were determined using quantitative RT-PCR. The effect of naringin and 5-fluorouracil combination significantly inhibited the growth and proliferation of the A375 cells in a concentration dependent manner with the IC50 values of naringin (24.75 μM) 5-fluorouracil (2.5 μM). The combination of naringin+5-fluorouracil on A375 cell lines at a concentration of half IC50 values (12µM+1 μM). Naringin and 5-fluorouracil combination also decreased the level of TNFα, IL-6, IL-1β, and NFκB mRNA in the A375 cell line. Naringin and 5-fluorouracil exerted anti-inflammatory effect through the suppression of NF-kB, IL-1β, TNFα, IL-6 in A375 cells. Taken together, our results suggested that treating A375 with naringin and 5-fluorouracil combination may have future applications in treating skin cancers through its anti-inflammatory effect.
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