SummaryIn this study the effect of different colchicine concentrations (0.025, 0.05, 0.1 and 0.2%) and treatment time (8, 24 and 48 h) on in vitro polyploidy induction from shoot tips was investigated as a factorial experiment based on completely randomized design with three replications. Polyploidy induction was confirmed by chromosome counts, size and number of stomata and other morphological characters. By assessment of different media, it was revealed that the suitable medium for regeneration of shoot tips is MS medium supplemented with 0.1 mg L 1 IBA (indole-3-butyric acid) and 1 or 2 mg L 1 BAP (6-benzylaminopurine) or 2 mg L 1 TDZ (thidiazuron). In addition, MS medium supplemented with 1 mg L 1 NAA (1-naphthaleneacetic acid) and 0.5 or 1 mg L 1 IBA led to the maximum rooting of plantlets. With increasing colchicine concentration and its treatment duration, explants survival and their rooting considerably decreased. The results revealed that 0.1% concentration of colchicine with 48 h of treatment established the maximum amount of the in vitro induced tetraploid plantlets. The derived tetraploid plantlets had bigger stomata with lower density. Chlorophyll content in tetraploid plantlets was significantly higher than diploids, and they also showed an elevated level of antioxidant enzymes, protein and soluble carbohydrate contents as compared with diploids.
Bride rose poppy (Papaver fugax Poir.) is a source of several pharmaceutical benzylisoquinoline alkaloids such as morphine and codeine. In order to determine the best method for the production of the autotetraploid population in this species, seeds and seedlings were treated with different colchicine concentrations (0, 0.05, 0.1, 0.2, and 0.5%) for 12, 24, and 48 h. Flow cytometry and chromosome observation from leaves confirmed the chromosome doubling in some of the treated plants. Chromosome doubled plants were studied in terms of morphological and physiological traits. The highest induction frequency of the tetraploid plants (18.35%) obtained by treatment of seeds or seedlings with 0.1% colchicine for 48 h. In induced tetraploid plants, the density of stomata, average internode length, and plant height decreased, while, stomata size, leaf thickness, length and width, number of leaves, fresh and dry weight of plants, capsule size, chlorophyll, protein, and carbohydrate contents and activity of catalase and peroxidase antioxidant enzymes increased.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.