Blood samples were obtained from selected and unrelated individuals. DNA was extracted with the standard Chelex® 100 (BioRad, Hercules, CA) extraction procedure (1); DNA samples were amplified in a DNA Gene Amp 9700 (Applied Biosystems, Foster City, CA) using 10 ng of template DNA. The amplified products were detected using the Abi Prism 310 Genetic Analyzer (Applied Biosystems, Foster City, CA).
We demonstrate here the successful use of laser capture microdissection (LCM) and DNA fingerprinting in the identification of a case of gastric bioptic specimen mix-up. A 70-year-old man, suffering from chronic atrophic gastritis, underwent to a gastric biopsy and received a diagnosis of gastric cancer. In the absence of any clinical evidence of gastric cancer, a specimen mix-up was suspected. LCM was used to retrieve gastric cells from the histologic slide, classified as gastric carcinoma, and suspected to be mislabelled. DNA was extracted from microdissected cells, and a total of 16 different genetic loci were analyzed, using an identity test. Comparison of the results with those obtained using DNA extracted from a control slide, and from patient's saliva, demonstrated a distinct DNA fingerprint pattern in all genetic markers examined, clearly indicating the occurrence of a specimen mix-up. The combined use of LCM and DNA fingerprinting represents the most accurate and sophisticated method available for the identification of specimen mix-up, especially when only the tissue on the suspected slide is available.
Blood samples were obtained from selected and unrelated individuals. DNA was extracted with the standard Chelex® 100 (Bio-Rad, Hercules, CA) extraction procedure (1); DNA samples were amplified in a DNA Gene Amp 9700 (Applied Biosystems, Foster City, CA) using 10 ng of template DNA. The amplified products were detected using the Abi Prism 310 Genetic Analyzer (Applied Biosystems, Foster City, CA).
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