Distribution of D5S818, D7S820, D8S1179, D13S317, D18S51 Alleles in a Central Italian Population Sample

Abstract

Blood samples were obtained from selected and unrelated individuals. DNA was extracted with the standard Chelex® 100 (BioRad, Hercules, CA) extraction procedure (1); DNA samples were amplified in a DNA Gene Amp 9700 (Applied Biosystems, Foster City, CA) using 10 ng of template DNA. The amplified products were detected using the Abi Prism 310 Genetic Analyzer (Applied Biosystems, Foster City, CA).

Allele frequencies for nine STR loci (D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820) in the Italian population

“…All the analyzed loci reached Hardy-Weinberg equilibrium in the population studied ( p > 0.05) and the allele frequencies calculated were not different from those observed in other areas of Italy [3][4][5][6][7].…”

“…The length of amplified DNA fragments was determined in comparison with an internal size standard GeneScan TM -500 ROX (Applied Biosystems). Each electrophoretic run was analyzed with GeneScan TM Analysis software v. 3 A total of 100 samples were re-tested blindly following our internal quality control program.…”