Candida species were isolated on Sabouraud's dextrose agar. Speciation of Candida was done by Dalmau plate technique on corn meal agar. Also, sugar assimilation and fermentation tests were performed by using 2% concentration of glucose, maltose, sucrose, lactose, galactose, and trehalose were used for test in which Bromothymol blue indicator was used [5].
Microbiology SectionComparison of Various Methods Used to Detect Biofilm Production of Candida Species ABSTRACT Purpose: The biofilm of organisms can be considered as a virulence factor because of the resistance towards antimicrobial agents. Formation of Candida biofilms are observed in blood, mucosal surface and most medical devices i.e. nonliving objects in patient's body. The study was undertaken to conclude the most sensitive and specific test for detection of biofilm formation.
Abstract:Background: Hepatitis B is a potentially life -threatening liver infection caused by the hepatitis B virus. It is a global health problem. Objectives: To estimate the Seroprevalence of HBsAg and its age and sex wise distribution in a hospital based population. Materials and Methods: Serum samples collected over a period of 12 months from patients attending OPDs and admitted to various IPDs of Krishna Hospital and Medical Research Center Karad, were included in the study. Seroprevalence of Hepatitis B surface antigen among hospital based general population was determined using a third generation ELISA. Statistical analysis: Percentages, chi square test. Results: A total number of 7373 patients were screened for HBsAg, among them 3238 (43.92%) were males and 4135 (56.08%) were females. The seroprevalence of HBsAg was 166(2.25%). The seroprevalence of HBsAg was higher in males 85(2.63%) as compared to females 81(1.96%). The highest seroprevalence was found to be among 51-60yrs age group (5.24%)
In the present study, significant risk factors were a history of blood transfusion, habit of alcohol, dialysis. The prevention of HCV infection can be achieved by screening of blood and blood products and creating awareness about risk factors. Since the efficacy of current and new therapies differ by genotype, genotype study is essential.
Introduction:The pathogenicity of Staphylococcus aureus depends on various bacterial surface components and extracellular proteins. S. aureus expresses a variety of virulence factors, including Panton-Valentine leukocidin (pvl). pvl is a cytotoxin produced by S. aureus that causes leukocyte destruction and tissue necrosis. Despite the presumed importance of pvl as a virulence factor, few data are available on its prevalence among S. aureus isolates in our area.
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