Comparison of Various Methods Used to Detect Biofilm Production of Candida Species

Dhale, Rahul P.; Mv, Ghorpade; Dharmadhikari, C A

doi:10.7860/jcdr/2014/10445.5147

Cited by 23 publications

(24 citation statements)

References 13 publications

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“…In the present study 100% of Candida isolates were found to be biofilm producers by XTT reduction assay. In a study by Dhale et al 16.94% isolates were detected as biofilm positive strains by XTT reduction assay [18], which is in contrast with the present study, but their findings with other methods were also low. Whereas in the present study all isolates were biofilm producers as detected by two different methods.…”

Section: Xtt Reduction Assaycontrasting

confidence: 93%

Detection of Biofilm Production and its Quantification in Candida Isolates in a Tertiary Care Hospital

Tiwari¹,

Mishra²,

Dogra³

et al. 2019

SJPM

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Introduction: Candida biofilms adversely impact the health of the patients with increasing frequency and severity of disease and with soaring economic sequel. Objective: Qualitative detection of biofilm production and its quantification was performed in Candida isolates from patients infected with health care associated infection (HCAI). Method: A total of 55 Candida isolates were included in the study. Biofilm production was estimated by Tube method (TM) and Tissue culture plate method (TCP). Further quantification of the biofilm produced was performed by XTT (2, 3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide) reduction Assay and Dry weight measurement method. Result: All Candida isolates were found to be biofilm producers by all three (TM, TCP and XTT) methods. Quantity of biofilm produced by C. albicans ranged between 2.3 to 9.1 mg/disk. Among non-albicans Candida Candida tropicalis) it was between 2.2 to 7.3 mg/disk whereas non-albicans Candida (except C. tropicalis) weight of the biofilm was 2.0 to 7.1 mg/disk. Conclusion: Dry weight (DW) is the actual quantity of biofilm produced. Candida albicans produced higher quantity of biofilm than non-albicans Candida in the study. It is also concluded that quantitative detection of biofilm is definitely help clinician in deciding modality of treatment.

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Section: Xtt Reduction Assaycontrasting

confidence: 93%

Detection of Biofilm Production and its Quantification in Candida Isolates in a Tertiary Care Hospital

Tiwari¹,

Mishra²,

Dogra³

et al. 2019

SJPM

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“…Biofilm formation on the surface of microplates and the antibiofilm effect of APDT were evaluated using a quantitative crystal violet assay as described previously (7). Briefly, the culture broth media in the wells of the microplates was aspirated gently, and wells were washed twice with distilled water.…”

Section: Photodynamic Treatment Of Biofilms and Antibiofilm Assaymentioning

confidence: 99%

The in vitro effect of antimicrobial photodynamic therapy on Candida and Staphylococcus biofilms

Tunçcan¹,

Kalkancı²,

Unal³

et al. 2018

Turk J Med Sci

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“…40 Briefly, overnight culture was adjusted to 100 lL 0.5 McF units (5 3 10 6 cells/mL) by using SDM (pH 5.6) and then transferred to one of the wells of the 96-well tissue culture plate (ThermoFisher Scientific, Nonclon) already containing 100 lL SDM (pH 5.6). The plate was incubated at 308C for 48 hours.…”

Section: Biofilm-forming Potential Of Ocular Isolates Of C Albicans mentioning

confidence: 99%

Temporal Expression of Genes in Biofilm-Forming Ocular Candida albicans Isolated From Patients With Keratitis and Orbital Cellulitis

Ranjith

Chakravarthy

Adicherla

et al. 2018

Invest. Ophthalmol. Vis. Sci.

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Citation: Ranjith K, Kalyana Chakravarthy S, Adicherla H, Sharma S, Shivaji S. Temporal expression of genes in biofilm-forming ocular Candida albicans isolated from patients with keratitis and orbital cellulitis. Invest Ophthalmol Vis Sci. 2018;59:528-538. https://doi.org/ 10.1167/iovs.17-22933 PURPOSE. To study antibiotic susceptibility and biofilm-forming potential of ocular isolates of Candida albicans along with gene expression. METHODS.Seven clinical isolates of C. albicans (keratitis-6 and orbital cellulitis-1) were evaluated. Biofilm formation in one isolate was monitored by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). Expression of 27 genes (real-time PCR) associated with biofilm formation and virulence was compared between biofilm-positive and biofilm-negative ocular C. albicans isolates. The temporal expression (4 to 72 hours) of the 27 overexpressed genes was also determined. Similar studies were also done with biofilmpositive and biofilm-negative nonocular C. albicans.RESULTS. Four of seven ocular C. albicans isolates exhibited the potential to form biofilm, one of which was resistant to three antifungals, whereas three were susceptible to all. SEM studies indicated that biofilm increased from two to three adherent layers of cells at 24 hours to multiple layers by 72 hours. CLSM showed that biofilm thickness increased from 5.2 lm at 24 hours to 17.98 lm at 72 hours. Upregulation of 27 genes involved in virulence and biofilm formation was observed both in the ocular and nonocular C. albicans positive for biofilm formation and compared to the respective non-biofilm-forming C. albicans. The results also indicated similarity in expression of genes between biofilm-forming ocular and nonocular pathogenic C. albicans. Temporal expression of the 27 genes (involved in adhesion, initiation, maturation, and dispersal stages of biofilm) in the biofilm-positive ocular isolate indicated that expression pattern followed four different patterns.CONCLUSIONS. This is the first study showing similarity in expression of genes in biofilmforming ocular and nonocular isolates of C. albicans, suggesting that upregulated genes could serve as a potential target for developing therapeutic strategies.

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Comparison of Various Methods Used to Detect Biofilm Production of Candida Species

Cited by 23 publications

References 13 publications

Detection of Biofilm Production and its Quantification in Candida Isolates in a Tertiary Care Hospital

Detection of Biofilm Production and its Quantification in Candida Isolates in a Tertiary Care Hospital

The in vitro effect of antimicrobial photodynamic therapy on Candida and Staphylococcus biofilms

Temporal Expression of Genes in Biofilm-Forming Ocular Candida albicans Isolated From Patients With Keratitis and Orbital Cellulitis

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