Mi.IX is a new phenotype in the Miltenberger series of the MNS blood group system with a frequency of 0.43% in Denmark. Mi.IX red cells are Mur+ but do not express any of the other established Miltenberger determinants. They react with a new antibody, anti-DANE, which defines a determinant present on Mi.IX cells but not on cells of other Miltenberger phenotypes. Four Mi.IX propositi have been found. Their families show that Mi^lx is inherited with a MS complex (lod score 3.69 at Θ = 0.00) which produces a trypsin-resistant M antigen. DANE has been allotted the ISBT number 002032 (MNS32). Serological and immunochemical studies with human and monoclonal antibodies to various determinants on glycophorin A (GPA) suggest that Mi.IX is associated with an aberrant GPA molecule that lacks the trypsin cleavage site at amino-acid residue 39, retains the chymotrypsin cleavage site at residue 34 and has an apparent Mr of about 1,000 less than normal GPA. It is proposed that this Mi.IX molecule has an amino acid and possibly also a glycosylation change in the region of amino-acid residues 35-39.
Immunoblotting was performed, after sodium dodecyl sulphate polyacrylamide gel electrophoresis of
solubilised red cell membranes under non-reducing conditions, with human Lutheran antibodies, anti-Lu^a, -Lu^b, -Lu3,
-Lu6 and -Lu8, and with human para-Lutheran antibodies, -Lu4, -Lu12 and -Lu17. On antigen-positive red cells all
antibodies revealed two components of apparent molecular weight (M(r)) 83,000 and 76,000. Anti-Lu^a and -Lu^b gave no
bands with Lu(a-b+) or Lu(a+b-) cells, respectively. With the exception of anti-Lu17, which reacted normally, and
anti-Lu3, which reacted weakly, none of the antibodies showed bands with membranes from Lu(a-b-) cells of the In(Lu)
type. Treatment of the red cells with sialidase led to a small reduction in the apparent Mr of the Lutheran glycoproteins
which, therefore, appear to contain sialic acid bearing O-linked oligosaccharides.
Immunoblotting was performed, after sodium dodecyl sulphate polyacrylamide gel electrophoresis of solubilised red cell membranes under non-reducing conditions, with human Lutheran antibodies, anti-Lua, -Lub, -Lu3, -Lu6 and -Lu8, and with human para-Lutheran antibodies, -Lu4, -Lu12 and -Lu17. On antigen-positive red cells all antibodies revealed two components of apparent molecular weight (Mr) 83,000 and 76,000. Anti-Lua and -Lub gave no bands with Lu(a-b+) or Lu(a+b-) cells, respectively. With the exception of anti-Lu17, which reacted normally, and anti-Lu3, which reacted weakly, none of the antibodies showed bands with membranes from Lu(a-b-) cells of the In(Lu) type. Treatment of the red cells with sialidase led to a small reduction in the apparent Mr of the Lutheran glycoproteins which, therefore, appear to contain sialic acid bearing O-linked oligosaccharides.
Mi.IX is a new phenotype in the Miltenberger series of the MNS blood group system with a frequency of 0.43% in Denmark. Mi.IX red cells are Mur+ but do not express any of the other established Miltenberger determinants. They react with a new antibody, anti-DANE, which defines a determinant present on Mi.IX cells but not on cells of other Miltenberger phenotypes. Four Mi.IX propositi have been found. Their families show that MiIX is inherited with a MS complex (lod score 3.69 at theta = 0.00) which produces a trypsin-resistant M antigen. DANE has been allotted the ISBT number 002032 (MNS32). Serological and immunochemical studies with human and monoclonal antibodies to various determinants on glycophorin A (GPA) suggest that Mi.IX is associated with an aberrant GPA molecule that lacks the trypsin cleavage site at amino-acid residue 39, retains the chymotrypsin cleavage site at residue 34 and has an apparent Mr of about 1,000 less than normal GPA. It is proposed that this Mi.IX molecule has an amino acid and possibly also a glycosylation change in the region of amino-acid residues 35-39.
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