Continuous culture of malignant haemic cells from human acute myelomonocytic leukaemia: cytological, cytochemical, cytogenetic and immunological studies

Karpas, Abraham; Khalid, Ghizala; Burns, Gordon F.; Hayhoe, F. G. J.

doi:10.1038/bjc.1978.40

Cited by 16 publications

(7 citation statements)

References 11 publications

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“…They were fixed in acetone and tested for the presence of EBNA, using the original method (Reedman & Klein, 1973). Our known EBNA positive lines (160 and 117) (Karpas et al, 1977) were used as positive controls and T-cells (line 45) (Karpas et a/, 1977) and myeloblasts (line 230) (Karpas et a / , 1978) were used as negative controls. The tests were performed on two occasions, the fresh material being tested on the same day that it was obtained and the cultured cells after 2 weeks in vitro.…”

Section: Immunological Surface Markersmentioning

confidence: 99%

Sinus Histiocytosis with Massive Lymphadenopathy: Virological, Immunological and Morphological Studies

et al. 1980

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The histiocytes from a patient with sinus histiocytosis were tested for the presence of Epstein-Barr viral nuclear antigen (EBNA) before and after in vitro growth. On both occasions the histiocytes as well as the lymphocytes were EBNA negative. Likewise the profile of EBV viral capsid antibody (VCA) suggests that this virus is unlikely to be directly involved in the development of the disease. The maintenance of an unusually high level of antibodies to EBV and measles, both viruses which produce persistent infections, indicates that the humoral immune response is active. That a normal humoral response occurred in response to mumps virus during the same period suggests that a deficiency in the cell mediated immune response to the persistent viruses might be the reason for the abnormally high antibody levels. The fresh as well as the cultured histiocytes had only Fc receptors. The affected lymph node lymphocytes developed unusual large inclusion bodies following in vitro culture.

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Section: Immunological Surface Markersmentioning

confidence: 99%

Sinus Histiocytosis with Massive Lymphadenopathy: Virological, Immunological and Morphological Studies

et al. 1980

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“…The following human haemic cell lines have been used for comparative studies in the terminal deoxynucleotidyl transferase (TdT) and alkaline phosphatase assays: (1) line 45: T-cell lymphoblasts (Karpas et al, 1977a), (2) line 230: myeloblasts (Karpas et al, 1978a).…”

Section: Cells Usedfor Comparative Studiesmentioning

confidence: 99%

“…In recent years there have been several reports of successful continuous culture of presumably malignant haemic cells from leukaemic patients. Most of these cell lines have been established from patients with acute lymphoblastic leukaemia (ALL), and include T-cells (Minowada et al, 1972;Minowada & Moore, 1975;Karpas et al, 1977a;Morikawa et al, 1978), null cells (Karpas et al, 1977b;Rosenfeld et al, 1977) and B-cell (Hiraki et al, 1977) lines. B-cell lines have also been established from lymphocytic lymphomas (Nilsson & Sundstrom, 1974) a histiocytic line from diffuse histiocytic lymphoma (Epstein & Kaplan, 1974).…”

mentioning

confidence: 99%

“…A few cell lines with malignant features have also been established from patients with myeloid leukaemia. These include two lines from patients with chronic granulocytic leukaemia (Lozzio & Lozzio, 1975;Minowada et al, 1977), two from patients with acute myeloid leukaemia (AML) (Collins et al, 1977;Kaeffler & Golde, 1978), and one from a patient with acute myelomonocytic leukaemia (AMML) (Karpas et al, 1978a). We now report the establishment of a cell line (241) from the peripheral blood of a patient with longstanding non-Hodgkin's lymphoma at a time when terminal transformation to an acute monocytic leukaemia had occurred.…”

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confidence: 99%

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Phenotypic Characterization of a Virus Producing Line Established from a Patient with Leukaemia/Lymphoma: Comparison with T‐Lymphoblastic and Myeloblastic Lines

Karpas¹,

Worman²,

Khalid³

et al. 1980

Br J Haematol

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The properties have been studied of a haemic cell line established from a patient with longstanding non-Hodgkin's lymphoma after transformation to acute leukaemia. The cells are EBNA, Fc, C3 and SmIg and Ia-like antigen negative. The continuous in vitro proliferation and karyotype abnormalities suggest malignancy. The cells contain only the lymphoid type of alkaline phosphatase and a high level of terminal deoxynucleotidyl transferase and as they formed E-rosettes during the first year in culture they may be of T-cell lineage. Since the patients' leukaemic cells have not been studied it is impossible to know whether the cultured cells represent an in vitro proliferation of the patient's malignant cells or an outgrowth of a subpopulation of cells. In the cytoplasm of many cells inclusion bodies containing virus-like particles, similar to those observed in fresh human leukaemia cells, were often seen. As this new virus is biologically active, infection of the cells by virus may explain their continuous proliferation in vitro.

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“…[12] and a primary hairycell culture [13] were investigated for Mk rosette formation. Before testing, the cells were washed in serum-free Hanks' balanced salt solution.…”

Section: Mk Rosettesmentioning

confidence: 99%

The detection of membrane‐associated complement components (C 3 and C 4) on circulating human normal and leukemic leukocytes and on cultured cells with monkey erythrocytes

Burns

Cawley

1979

Eur J Immunol

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Monkey erythrocyte (Mk) rosette formation is described as an exquisitely sensitive method for the detection of complement (C) components on the membrane of human leukocytes. Blocking of the immune adherence receptor on Mk blocked subsequent rosette formation as did pretreatment of leukocytes with antiserum to the C components C 3 and C 4. In vitro C deposition by immune complex formation with normal human lymphocytes enhanced Mk rosette formation, and this could be inhibited with antiserum to C 3. The use of Mk rosette formation revealed that cells from a wide variety of human lymphoid and myeloid leukemias carry membrane-bound C. It was also shown that several lymphoblastoid cell lines, including a T cell line, probably synthesize both C 3 and C 4. Mk rosette formation is not dependent on metabolic activity of the rosetting leukocyte, and it is suggested that this technique will be of value in detecting C deposition in a variety of situations.

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Continuous culture of malignant haemic cells from human acute myelomonocytic leukaemia: cytological, cytochemical, cytogenetic and immunological studies

Abstract: Images Fig. 1 Fig. 2 Fig. 3

Cited by 16 publications

References 11 publications

Sinus Histiocytosis with Massive Lymphadenopathy: Virological, Immunological and Morphological Studies

Sinus Histiocytosis with Massive Lymphadenopathy: Virological, Immunological and Morphological Studies

Phenotypic Characterization of a Virus Producing Line Established from a Patient with Leukaemia/Lymphoma: Comparison with T‐Lymphoblastic and Myeloblastic Lines

The detection of membrane‐associated complement components (C 3 and C 4) on circulating human normal and leukemic leukocytes and on cultured cells with monkey erythrocytes

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