An optimized and validated spectrophotometric method has been developed for the determination of Tetracycline hydrochloride (TCH) in pharmaceutical formulations. The method is based on the reaction between TCH, sodium nitroprusside and hydroxylamine hydrochloride in alkaline medium. The product absorbed maximally at 529 nm. Beer's law is obeyed in the working concentration range of 2-60 µg mL-1 with apparent molar absorptivity of 5.049×10 3 L mol-1 cm-1 and Sandell's sensitivity of 0.0952 µg cm 2 / 0.001 absorbance unit. The proposed procedure was applied successfully for estimation of drug in different commercial forms. The proposed method was applied to the determination of the studied drug in pharmaceutical formulations and the results demonstrated that the method is equally accurate and precise as the official method as found from the t-and F-values.
A simple, rapid and sensitive spectrophotometric method for the determination of baclofen in pharmaceutical preparations were developed by combining a spectrophotometric detector with Flow injection analysis. This method based on the oxidation of iron (II) to iron (III) by baclofen and formation of complex between iron (III) and potassium hexacyano ferrate (II) in acidic solution. The absorbance from prussian blue dye is recorded at 700 nm. Chemical and physical parameters of this system were investigated. The linearity of baclofen is ranged from (0.05-25) mmol.L -1 with correlation coefficient r = 0.9972. The limit of detection (S/N=3) was equaled 0.01 mmol.L -1 the L.O.Q was 1.05 mmol.L -1 and a relative standard deviation for 6 replicate determinations of baclofen in 10 mmol.L -1 solution was 1.3 % using 100 µL sample volume. The proposed method was successfully applied to the determination of baclofen in pharmaceutical formulation with recovery 99%. Using paired t-test it was shown that there was no significant difference between the proposed method and official method on that basis the proposed method can be accepted as an alternative analytical method.
A fast and sensitive flow injection analysis (FIA) method for the quantitative determination of thymol (THY) in raw and pharmaceutical formulations have been proposed. The method is based on coupling reaction between THY and 2,4-dinitrophenylhydrazine in alkaline medium with presence of potassium periodate to form an intense violet water-soluble dye that is stable and has a maximum absorption at 570 nm. A graph of absorbance versus concentration indicates that Beer's law is obeyed over the concentration range of 10-150 μg.mL-1 of THY, with detection limit of 5 μg.mL-1 and sample throughputs of 60h-1. The proposed method was successfully applied to the determination of THY in mouth wash preparations.
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