Sensitive Spectrophotometric Determination of Tetracycline Hydrochloride Indosage forms using Sodium Nitroprusside and Hydroxylamine Hydrochlorid

Hadi, Hind; Fadhil, Ghadah

doi:10.22401/jnus.17.3.07

Cited by 6 publications

(5 citation statements)

References 10 publications

(8 reference statements)

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“…It was indicated that NaOH gave maximum colour intensity in 385 nm Figure 2. The results were compatible with the previous research which indicate that the reaction can be done in a basic medium [30,31]. 3.…”

Section: Type Of Basesupporting

confidence: 93%

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Using 9-Chloroacridine as A Chromogenic Reagent for The Detection of Tetracycline

2021

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An accurate, cost-effective and simple method has been described for the quantitative determination of Tetracycline hydrochloride (TCs) in bulk and pharmaceutical samples using an aqueous medium. The proposed method is based on the nucleophilic substitution reaction of 9-chloroacridine (9-CA) as a chromogenic reagent with TCs in an aqueous solution forming a colored complex. The spectrum of the product shows maximum absorption at 385 nm against the blank. The linearity range of the calibration graph was (1-28) μg.mL -1 with a limited detection value (LOD) 0.138 μg.mL -1 . The results show the absence of interferences from the excipients compounds on the determination of the drug. The suggested method exhibited high reproducibility (RSD) 2.3 % with mean percentage recovery (R%) 99.95. Furthermore, the stability constant has been calculated to be 9.843×10 5 L.mol -1 and the reaction mechanism is proposed. A ratio of 1: 1 was found between the above drug and the reagent using Job and molar ratio methods. The accuracy and validity of the proposed method were further determined by performing a recovery experiment by the use of the standard addition method. It is found that the method does not require extraction process and it agree well standard addition method. Application of the suggested method to commercial pharmaceutical preparations (capsule and ointment) is presented.

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Section: Type Of Basesupporting

confidence: 93%

“…Comparing the obtained resulted with published work [5,30,31,35]. As can be seen in Table 10, the proposed method has higher sensitive than the cited works.…”

Section: Figure 9 Standard Addition Curve To Determine Tcs Capsulementioning

confidence: 61%

Using 9-Chloroacridine as A Chromogenic Reagent for The Detection of Tetracycline

2021

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“…found is 0.0125 μg/mL and the limit of quantification (LOQ) is 0.0412 μg/mL. This LOQ obtained is approximately 35 times lower than that (1.739 μg/mL) reported for the spectrophotometric method [3]. It is close (0.026 μg/mL) to that reported for HPLC [6].…”

Section: Discussionsupporting

confidence: 47%

“…A large number of analytical techniques for the determination of tetracycline and its degradation products have been reported, particularly in biological fluids and pharmaceuticals such as spectrophotometry [2] [3] which is of limited utility due to its non-specificity. More efficient separative methods, such as capillary electrophoresis [4] and high performance liquid chromatography [5] [6] using UV and fluorescence detectors require a lot of time, proven expertise and a very high operating cost.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of a Spectrofluorimetric Method for the Assay of Tetracycline in Capsules

Namegabe¹,

Sarr²,

Diop³

2018

AJAC

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The purpose of this study is to develop and validate a method for the analysis of tetracycline capsules by spectrofluorimetry. A pH 9 borate buffer was used as diluent of tetracycline after reaction with magnesium salt at the excitation wavelength of 372 nm and 516 nm of emission. A linear response was observed between 0.25 μg/mL and 1.5 μg/mL with a correlation coefficient (R) of 0.9998. The detection and quantification limits found are 0.0125 μg/mL and 0.0412 μg/mL respectively. The proposed method proved trueness with a recovery between 99.88% and 101.10%. The relative standard deviations of repeatability and intermediate precision found ≤2.88% reflected a good precision of the method. The proposed method is therefore valid within the limits of 90% to 110%. The proposed method was applied to the quality control of 9 tetracycline samples from market and gave results in accordance with the pharmacopoeia standards.

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“…All different concentrations SSAS of tetracycline antibiotic used in this research were prepared from dilution of the stock solution with distilled water to the desired concentration. Tetracycline concentrations were determined spectrometrically at a wavelength of λ=529 nm using a spectrophotometer according to the method described by (12) . Figure 1 shows the spectrophotometer calibration curve intended for tetracycline concentrations.…”

Section: Stock Solutionsmentioning

confidence: 99%

Removal of Tetracycline from Aqueous Solutions using Pomegranate Peels Residues Accessing to ZRL

2020

IJFMT

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Antibiotics are harmful pharmaceuticals to ecosystems in general and aquatic systems in particular. Therefore, its remediation from water bodies is a topic of great importance for water treatment and purification workers. This research investigates the removal of one of the most famous types of antibiotics, which was tetracycline from simulated synthetic aqueous solutions by adsorption technique using non-toxic, low cost and available agricultural waste which was pomegranate peels. The adsorption experiments were performed in adsorption laboratory unit of batch mode at different operating conditions and laboratory temperature. The operating parameters studied included pH of solution, dose of adsorbent media, treatment time, agitation speed and initial concentration of tetracycline. The results showed the ability of pomegranate peels to extract tetracycline from aqueous solutions with high efficiency of 81.55%. The results also showed that the percentage of antibiotic adsorption from aqueous solutions was inversely correlated with increasing the initial concentration and acidic function of the tetracycline solution while it was directly proportional to the amount of pomegranate peels, agitation speed and treatment time. In this style, one of the most important types of antibiotics that contaminated water was disposed of by a cheap material and using a simple, economical and environmentally friendly method accessing to the principle of zero residue level (ZRL).

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Sensitive Spectrophotometric Determination of Tetracycline Hydrochloride Indosage forms using Sodium Nitroprusside and Hydroxylamine Hydrochlorid

Cited by 6 publications

References 10 publications

Using 9-Chloroacridine as A Chromogenic Reagent for The Detection of Tetracycline

Using 9-Chloroacridine as A Chromogenic Reagent for The Detection of Tetracycline

Development and Validation of a Spectrofluorimetric Method for the Assay of Tetracycline in Capsules

Removal of Tetracycline from Aqueous Solutions using Pomegranate Peels Residues Accessing to ZRL

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