As plantas têm sido utilizadas como um importante recurso para sociedade, por possuir propriedades biológicas e químicas. Espécies vegetais oleaginosas estão sendo alvo de diversos trabalhos para estudar o potencial de seus extratos como matéria-prima para elaboração de diversos produtos. Entre essas espécies se destaca a pupunheira (Bactris gasipaes, Kunth). O presente trabalho teve por objetivo avaliar o potencial biológico de diferentes extratos oriundos de frutos da pupunha. Os extratos da pupunha foram obtidos a partir de extratores do tipo Soxhlet, utilizando metanol e hexano como solvente. Os maiores rendimentos obtidos foram de cerca de 27% a 25% extratos para a extração da casca e da polpa do fruto respectivamente. A atividade antimicrobiana foi avaliada em cepas de Escherichia coli (ATCC25922), Staphylococcus aureus (ATCC25923) e Klebsiella pneumoniae (ATCC700603), por meio da técnica de disco difusão. Foram obtidos resultados negativos para algumas amostras de extratos do caroço e positivos para polpa e da casca. Os resultados obtidos demonstram potencial para a presença de substâncias nos extratos analisados com potencial atividade antioxidante e antimicrobiana na pupunha e estes resultados colaboram para novas investigações, e formulações de produtos biotecnológicos envolvendo extratos da pupunha amazônica. Palavras-chave: Perfil Cromatográfico. Atividade Antimicrobiana. Pupunheira. DPPH/TLC. Abstract Plants have been used as an important resource for the society, as they have biological and chemical properties. Oilseed plant species are the target of several studies that evaluate the potential of their extracts as raw material for various products. Among these species, the peach palm (Bactris gasipaes, Kunth) stands out. The present work aimed to evaluate the biological potential of different extracts from pupunha fruits. The pupunha extracts were obtained from Soxhlet extractors, using methanol and hexane as solvent. The highest yields obtained were about 27% to 25% extracts for the extraction of the peel and pulp of the fruit respectively. Antimicrobial activity was evaluated in strains of Escherichia coli (ATCC25922), Staphylococcus aureus (ATCC25923) e Klebsiella pneumoniae (ATCC700603), using the disk diffusion technique. Negative results were obtained for some samples of core extracts and positive for pulp and peel, after taking the mean and standard deviation of the diameters of the formed halos . The results obtained demonstrate the potential for the presence of substances in the analyzed extracts with potential antioxidant and antimicrobial activity in pupunha and these results collaborate for further investigations, and formulations of biotechnological products involving extracts from the Amazonian pupunha. Key words: Cromatography Profile. Antimicrobial Activity. DPPH/TLC
Resistance to antimicrobial drugs has become a serious public health problem due to indiscriminate use by humans. Therefore, several alternatives have been studied to contain super microorganisms that cause diseases, among them the use of secondary compounds produced by plants together with endophytic microorganisms. Therefore, this work aimed to isolate, identify and analyze the antimicrobial potential of endophytic microorganisms of Piper hispidinervum C. DC. against pathogens that affect humans and different animals. First, the leaf and inflorescence were collected and the plant material was identified. The material collected went through the process of asepsis and inoculation, which resulted in the growth of endophytes. After their growth, several replications were performed to purify the colonies. Regarding the macromorphology of endophytic fungi, different textures and colors were observed. Were obtained 27 isolates of fungal cultures, among them the most found genera are Colletotrichum sp., and Fusarium sp., we also identified a species of Curvularia sp. The rest of the isolates were classified as Mycelia sterilia. For the antimicrobial susceptibility tests, the inoculum of the test pathogens was carried out in Mueller-Hinton culture medium and the suspension was adjusted to the MacFarland scale. To obtain the fungal extract, colony fragments were deposited in Erlenmeyer flasks with Potato-Dextrose medium, after 14 days the culture medium was filtered, then Ethyl Acetate was added to extract metabolites. The fungal extract obtained by Ethyl Acetate was used in the sensitivity test by the well diffusion method. The Fusarium Extract sp. esp07 was the most effective against the microorganisms tested, being able to inhibit S. aureus, E. coli and C. albicans, resulting in halos with an average of 23 mm, 21 mm and 14 mm, respectively. The Fusarium sp. esp04 and Curvularia sp. fol50 have also been shown to be effective against E. coli.
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