Recently the high transfection potential of the cationic polyfold increased transfection efficiency. This activity depends mer polyethylenimine (PEI) was described (Boussif O et al. on ligand-receptor interaction and was observed also at Proc Natl Acad Sci USA 1995; 92: 7297-7301

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Vaccination using well-characterized allogeneic tumor cell lines expressing standardized doses of immunostimulatory cytokines is an attractive alternative for autologous gene-transfected tumor cell vaccines. In the present study, we show that vaccination with irradiated allogeneic K1735 (H-2 k ) or B16F10 (H-2 b ) melanoma cells induces a moderate degree of cross-protection against the M-3 melanoma (H-2 d ) in DBA/2 mice. Cross-protection against the syngeneic tumor was markedly improved when the allogeneic vaccines were transfected with the interleukin-2 (IL-2) gene. The IL-2 gene-modified allogeneic vaccines were effective for prophylactic vaccination against subsequent tumor challenge and for therapeutic vaccination against pre-existing tumor deposits, with efficacies that were comparable with that of the IL-2 gene-modified syngeneic vaccines. Cross-protection correlated with the cytotoxic activity of splenocytes against M-3 targets. Allogeneic vaccination was not effective in another model, against the B16F10 melanoma in C57BL/6 mice, irrespective of genetic modification with the IL-2 or granulocyte-macrophage colony-stimulating factor genes. Cancer Gene Therapy (2000) 7, 870 -878

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