Coupling of cell-binding ligands to polyethylenimine for targeted gene delivery

Abstract

Recently the high transfection potential of the cationic polyfold increased transfection efficiency. This activity depends mer polyethylenimine (PEI) was described (Boussif O et al. on ligand-receptor interaction and was observed also at Proc Natl Acad Sci USA 1995; 92: 7297-7301

Transfection of large plasmids in primary human myoblasts

“…Neither of these agents enhanced PEI adenofection, possibly because the endosomes were already well lysed by the adenovirus before lysosome/endosome acidification or later by the osmotic shock of proton absorption by PEI upon endosome/lysosome acidification. Kircheis et al 44 have also observed that PEI or ligand-PEI-mediated transfection was not dependent on the presence of additional endosomolytic agents and Ogris et al 45 have shown that chloroquine enhanced transfection with PEI or transferrin-PEI only when complexes of a certain size were formed.…”

Intracellular trafficking of nonviral vectors

“…31 Antibodies generated against certain cell-specific receptors also have been used to direct gene transfer. Antibody-directed molecular conjugates have been targeted to: CD3, [32][33][34][35] CD5, 36 EGF-R, 37,38 and ErbB2. 39 Key to the efficacy of an antibody-directed molecular conjugate, however, is the ability of the antibody to trigger receptor internalization.…”

Protective copolymers for nonviral gene vectors: synthesis, vector characterization and application in gene delivery

“…A transferrin-PEI conjugate with one transferrin molecule per PEI molecule was prepared essentially as described. 26 For most studies, PEI polyplexes 8 were used at an N/P ratio of 8.…”