Bacillus thuringiensis (Bt) is a bacterial genus known mainly for its capability to synthesize, in addition to spores, a parasporal body or crystal (δ––endotoxin), comprised of proteins which are toxic to pest insects, i.e., Coleoptera, Diptera and Lepidoptera, and other orders. As well as plant pathogenic nematode and many other applications as endophytic plant growth promoting bacteria, or cleaning enviornmental form some chemicals polluting agents, and even in human medicine for cancer prevention. The Bt coexists in a close relationship with insects to which eventually infects and poisons. It is likely that these unfavorable changes occurred during the evolutionary pathway in the interactions Bacillus–insect, which resulted in the present biochemical diversity between these species. The goal of this minireview is to show role of Bt in comparation with other genus Bacillus and explain why Bt is of interest for biological control of pests in conventional, protected and organic agriculture regarding other biotechnological applications to fully exploit the beneficial potential of Bt
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