Bacillus thuringiensis (Bt) is more than a special agent for biological control of pests

Sanchez–Yáñez, Juan Manuel; Rico, José Luis; Ulíbrri, Gérard

doi:10.15406/jabb.2022.09.00282

Cited by 4 publications

References 107 publications

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Selectivity of Bacillus thuringiensis (Bacillales: Bacillaceae) to the polyphagous predator Ceraeochrysa claveri (Navás, 1911) (Neuroptera: Chrysopidae)

Daquila

Moi

Scudeler

et al. 2022

Preprint

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Biopesticides based on Bacillus thuringiensis (Bt) are widely used to control agricultural pests. These products are efficient and have high specificity to target insects. However, there are concerns about their impact on non-target organisms. Ceraeochrysa claveri, is a polyphagous predator with important value in the biological control of insect pests in crops of economic interest. This study aimed to analyze the effects of B. thuringiensis serovar aizawai CG-91 (Bta) on the post-embryonic development of C. claveri. Neonate larvae of C. claveri were fed with Diatraea saccharalis eggs contaminated with different concentrations of Bta (0.62, 1.25, 2.50, and 5.00 g.L-1) until your pupation. Samples of the midgut larvae were collected for histological and ultrastructural analysis, and the larval and pupal inviability data were collected at the end of the respective developmental stages. After the emergence of adult insects, were considered inviable those with morphological alterations. To evaluate the reproduction, adult insects couples were formed, and the oviposition was collecteds. No significant differences in the viability of eggs, larvae, pupae, and adults of C. claveri contaminated with Bta were reported. Midgut analyses did not reveal cellular damage in the epithelium, indicating a possible lack of insecticidal proteins receptors. The oviposition and their viability were similar between treated and control groups. Our results demonstrated that Bta did not affect the post-embryonic development of C. claveri. This illustrates that the combined use of Bta and C. claveri for the biological control of arthropod pests can be a sustainable strategy for integrated pest management.

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Selectivity of Bacillus thuringiensis (Bacillales: Bacillaceae) to the polyphagous predator Ceraeochrysa claveri (Navás, 1911) (Neuroptera: Chrysopidae)

Daquila

Moi

Scudeler

et al. 2022

Preprint

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Protatrans – growth biostimulants for centomopathogenic bacteria <i>Bacillus thuringiensis</i>

Adamovich,

Vyatchina,

Rubanenko

et al. 2024

Izvestiâ vuzov. Prikladnaâ himiâ i biotehnologiâ

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The study investigates the use of protatran compounds as growth stimulators for Bacillus thuringiensis bacteria, which are widely used as producers of biopesticides. Cultivation of the Bacillus thuringiensis strain subsp. kurstaki was carried out in a Luria-Bertani (LB) liquid medium. Protatrans (2-Me-C6H4OCH2COO- were added to the NN+(CH2CH2OH)3 (1), 4-Cl-C6H4 -SCH2COO-NN(CHCHOH) (2) and 4-Cl-CSOCHCOO NN+(CH2CH2OH)3 (3) media in concentrations of 1×10-4–1×10-8wt %. The LB medium without the addition of compounds 1–3 was used as a control. Cultures were incubated at a temperature of 30°C for 24 hours. The number of Bacillus thuringiensis cells was determined by serial dilution. The maximum growth was observed in a medium containing 1×10-4wt % of protatran 3. The number of cells was almost 10 times (966.7%) higher than in the control. In media with 1×10-5, 1×10-6, 1×10-7 and 1×10-8 wt % of compound 3, the number of cells was 4–7 times higher than in the control (by 371.7–666.7%). Protatrans 1 and 2 had a positive effect on Bacillus thuringiensis, increasing the number of cells by 83–292% compared to control. Therefore, it was demonstrated for the first time that commercially available non-toxic protatran compounds in microconcentrations are powerful growth stimulators for the entomopathogenic bacteria Bacillus thuringiensis. This indicates the potential for significant improvement and cost reduction of biotechnology for the production of bacterial insecticides based on Bacillus thuringiensis, used in agriculture, forestry and homesteads to control harmful insects.

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The Potential of a Novel Concept of an Integrated Bio and Chemical Formulate Based on an Entomopathogenic Bacteria, Bacillus thuringiensis, and a Chemical Insecticide to Control Tomato Leafminer, Tuta absoluta ‘(Meyrick)’ (Lepidoptera: Gelechiidae)

Mashtoly

El-Beltagi

Almujam³

et al. 2022

Sustainability

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Tomato leafminer, Tuta absoluta (Meyrick), (Lepidoptera: Gelechiidae), poses severe threats to sustainable tomato production globally with a high capacity to develop resistance to pesticides. Recently, the management programs for this cosmopolitan pest have relied on combinations of chemical insecticides which exacerbate the problems of cross-resistance and adverse environmental effects. A novel concept of integrated formulates through combining a chemical insecticide model of lambda-cyhalothrin with Bacillus thuringiensis (Bt) products as the formulation core was explored and evaluated. The susceptibility of the second instar larvae of tomato leafminer to the original formulation of lambda-cyhalothrin, Dipel, XenTari, and Agree products was assessed and compared to the combined formulations. The positive and negative impacts of lambda-cyhalothrin on the viability and pathogenicity of Bt strains were tracked and documented. The physicochemical properties of the combined formulates were examined and compared to the international standards of pesticide formulations. The proposed integrated combined formulates of Dipel, XenTari, and Agree biopesticides with lambda-cyhalothrin showed an enhancing effect and had a higher potential than the originally manufactured formulations alone for about a 3.67–10.08-fold impact on larval mortality. Lambda-cyhalothrin had no significant adverse effect on either the viability of Bt strains or the physicochemical properties of combined co-formulates. Therefore, such integrated combined formulates would have the potential to be involved as an alternative or a complementary approach in pest management and pest resistance management tools for sustainable pest management.

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Bacillus thuringiensis (Bt) is more than a special agent for biological control of pests

Cited by 4 publications

References 107 publications

Selectivity of Bacillus thuringiensis (Bacillales: Bacillaceae) to the polyphagous predator Ceraeochrysa claveri (Navás, 1911) (Neuroptera: Chrysopidae)

Selectivity of Bacillus thuringiensis (Bacillales: Bacillaceae) to the polyphagous predator Ceraeochrysa claveri (Navás, 1911) (Neuroptera: Chrysopidae)

Protatrans – growth biostimulants for centomopathogenic bacteria <i>Bacillus thuringiensis</i>

The Potential of a Novel Concept of an Integrated Bio and Chemical Formulate Based on an Entomopathogenic Bacteria, Bacillus thuringiensis, and a Chemical Insecticide to Control Tomato Leafminer, Tuta absoluta ‘(Meyrick)’ (Lepidoptera: Gelechiidae)

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