Nonlinear microscopy has evolved over the last few decades to become a powerful tool for imaging and spectroscopic applications in biological sciences. In this study, i 2 PIE, a novel spectral phase control technique was implemented in order to compress broad bandwidth supercontinuum light pulses generated in an All Normal Dispersion (ANDi) Photonic Crystal Fiber (PCF). The technique, based on time-domain ptychography, is here demonstrated in a nonlinear microscopy application for the first time. The first real-world application of this technique for second harmonic generation and two photon excitation fluorescence microscopies in biological samples is presented. We further show that in our implementation, i 2 PIE leads to improved contrast and signal-to-noise ratios in the generated images, compared to conventional compression techniques used in nonlinear microscopy.
A radiofrequency (RF) electromagnetic radiation safety survey had been carried out at public access points in 46 towns with 76 Global Systems for Mobile communication cell sites in two major cities in Ghana. The objective was to determine the levels of RF field in residential areas, schools and market places, and compare the measured results with the guidelines set by the International Commission of Non-Ionising Radiation (ICNIRP). Measurements were made with log-periodic antenna coupled with spectrum analyzer. The results varied from 0.85 to 1.07 mW m(-2) and 0.78 to 1.19 mW m(-2) for the transmission frequencies of 900 and 1800 MHz, respectively. The result generally shows a compliance with the ICNIRP limit of 0.024 % but was 108 times higher than a similar survey carried out in Ghana 2 y ago.
Embryo quality is a crucial factor affecting live birth outcomes. However, an accurate diagnostic for embryo quality remains elusive in the in vitro fertilization clinic. Determining physical parameters of the embryo may offer key information for this purpose. Here, we demonstrate that digital holographic microscopy (DHM) can rapidly and non-invasively assess the refractive index of mouse embryos. Murine embryos were cultured in either low- or high-lipid containing media and digital holograms recorded at various stages of development. The phase of the recorded hologram was numerically retrieved, from which the refractive index of the embryo was calculated. We showed that DHM can detect spatio-temporal changes in refractive index during embryo development that are reflective of its lipid content. As accumulation of intracellular lipid is known to compromise embryo health, DHM may prove beneficial in developing an accurate, non-invasive, multimodal diagnostic.
Embryo quality is a crucial factor affecting live birth outcomes. However, an accurate diagnostic for embryo quality remains elusive in the in vitro fertilization clinic. Determining physical parameters of the embryo may offer key information for this purpose. Here, we demonstrate that digital holographic microscopy (DHM) can rapidly and non-invasively assess the refractive index of mouse embryos. We showed that DHM can detect spatio-temporal changes in refractive index during embryo development that are reflective of its lipid content. As accumulation of intracellular lipid is known to compromise embryo health, DHM may prove beneficial in developing an accurate, non-invasive, multimodal diagnostic.
We present a novel nonlinear microscopy modality using a time-domain ptychographic phase measurement, i2PIE, to compress 80 MHz supercontinuum pulses from an ANDi PCF used as excitation source, improving contrast at reduced average power.
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