In continuation of our search for new bioactive compounds from soil microbes, a fluorescent Pseudomonas strain isolated from paddy field soil of Kuttanad, Kerala, India was screened for the production of bioactive secondary metabolites. This strain was identified as Pseudomonas mosselii through 16S rDNA gene sequencing followed by BLAST analysis and the bioactive metabolites produced were purified by column chromatography (silica gel) and a pure bioactive secondary metabolite was isolated. This bioactive compound was identified as Pseudopyronine B by NMR and HR-ESI-MS. Pseudopyronine B recorded significant antimicrobial activity especially against Gram-positive bacteria and agriculturally important fungi. MTT assay was used for finding cell proliferation inhibition, and Pseudopyronine B recorded significant antitumor activity against non-small cell lung cancer cell (A549), and mouse melanoma cell (B16F10). The preliminary MTT assay results revealed that Pseudopyronine B recorded both dose- and time-dependent inhibition of the growth of test cancer cell lines. Pseudopyronine B induced apoptotic cell death in cancer cells as evidenced by Acridine orange/ethidium bromide and Hoechst staining, and this was further confirmed by flow cytometry analysis using Annexin V. Cell cycle analysis also supports apoptosis by inducing G2/M accumulation in both A549 and B16F10 cells. Pseudopyronine B treated cells recorded significant up-regulation of caspase 3 activity. Moreover, this compound recorded immunomodulatory activity by enhancing the proliferation of lymphocytes. The production of Pseudopyronine B by P. mosselii and its anticancer activity in A549 and B16F10 cell lines is reported here for the first time. The present study has a substantial influence on the information of Pseudopyronine B from P. mosselii as potential sources of novel drug molecule for the pharmaceutical companies, especially as potent antimicrobial and anticancer agent.

Camptothecin is an important source for the synthesis of some of the major anti-cancer agents such as irinotecan and topotecan. Traditional source of camptothecin are prominently woody plants such as Camptotheca acuminata Decne. and Nothopodytes nimmoniana (Graham) Mabb., and the increasing demand for camptothecin leads to the level of threatening their existence. Ophiorrhiza species composed of herbaceous plants with quick growth characteristics which are reported as alternative source of camptothecin. The present review focus on taxonomical status, traditional uses, biological activities and phytochemical constituents with a special attention in bioproduction of camptothecin from Ophiorrhiza species and its future prospects.

In this study, Doxorubicin is used as an effectual induction of cardiotoxicity and heart malfunction in the taken experimental rats which is an anthracycline chemotherapeutic mediator used to the treat of a wide variety of malignancies. To examine the shielding property of ethanolic lower extract of clitoraternatea (EECT) scheduled doxorubicin(DOX) persuaded cardiotoxicity via albino wistar rats,through inspect the enzymatic,non-enzymatic antioxidant position,serum enzyme and Histopathology tissue.Experimental rats been provoked cardiotoxicity by means of a generous single dose of doxorubicin (15mg /body weight ) and treated orally through an ethanolic extract of clitoraternatea (EECT) for 14 days (100 mg/kg bw, 200 mg/kg bw and 300mg/kg bw,n=5). On 15 days, the rats are sacri iced by treated through Ketamine -Xylazine,Biochemical as well as histological remarks of the heart tissues had carry out. Cardiotoxicity had been calculated throughout to establish the cardio marker enzyme levels such as (LDH) Lactic acid dehydrogenase,(CK-MB) Creatinin kinase and (AST) Aspartate transaminase at the inish of the study. Management through EECT of 200mg/kg and 300mg/kg considerably reduced the levels of cardiac marker enzymes(p<0.05) and as well minimize the decrease of weight of the heart of DOX treated group. In heart tissue superoxide dismutase, glutathione and catalase heights be signi icantly greater than before and lipid oxide peroxide (LPO) signi icantly decreased in the extract (200 &300 mg /kg bw).

Camptothecin (CPT), a cytotoxic quinoline indole alkaloid, is an anticancer compound. Its two major semi synthetic derivatives, topotecan and irinotecan, are Food and Drug Administration (FDA) approved drugs effectively used for treating different cancer types and sold under the trade names Hycamtin and Camptosar. Among the Ophiorrhiza species, Ophiorrhiza mungos contains the highest CPT level (0.02 % g dw). CPT level was determined in plants before flowering (0.074 ± 0.003 % g dw) and at flowering (0.052 ± 0.002 % g dw). Multiple shoot cultures were induced on seedling-derived explants of O. mungos in half strength MS solid media supplemented with 1.0 mg/L BAP to obtain 12.00 ± 1.22 shoots in 20 days. Repeated subcultures at 20 days interval yielded 20.00 ± 3.71 shoots/subculture of shoot clusters. After elongation, rooting and transplanting, the growth of shoot clusters were studied in both in vitro and same aged naturally grown seedlings. Highest biomass (4.62 ± 0.158 g fw) was obtained in in vitro-derived shoot clusters. CPT increased according to biomass and the maximum CPT was recorded in in vitro rooted shoot clusters after 15 days (0.031 ± 0.001 % g dw). Hence, in vitro-derived rooted shoot clusters of O. mungos cultivated in net-pots for 60 days under shade net house conditions found to be a sustainable source for CPT.