Ophiorrhiza, a promising herbaceous source of the anticancer compound camptothecin

Krishnakumar, Gopal Shankar; Dintu, K. P.; Varghese, Sibi Chirakkadamoolayil; Nair, Deepthi S.; Gopinath, Geethu; Rameshkumar, K. B.; Satheeshkumar, K.; Krishnan, P. N.

doi:10.14719/pst.2020.7.2.660

Cited by 6 publications

(3 citation statements)

References 55 publications

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“… Loganiaceae Whole plant 0.01% (Dai et al 1999 ) Pyrenacantha klaineana Pierre ex Exell & Mendonça Icacinaceae Stem 0.00048% (Zhou et al 2000 ) Fruit 0.488% (Ramesha et al 2013 ) Ixora coccinea L. Rubiaceae Young leaves 0.4146 µg g −1 (Saravanan and Boopalan 2011 ) Mature leaves 5.0611 µg g −1 Ophiorrhiza. alata Craib Rubiaceae Leaves 83 µg g −1 (Krishnakumar et al 2020 ) Root 388 µg g −1 Ophiorrhiza rugosa var. decumbens (Gardner ex Thwaites) Deb & Mondal Rubiaceae Whole plant 4.20 µg g −1 (Krishnakumar et al 2020 ) Shoot 2 µg g −1 Root 24 µg g −1 Ophiorrhiza rugosa var.…”

Section: Camptothecin: Structure and Its Derivatives/analoguesmentioning

confidence: 99%

“…alata Craib Rubiaceae Leaves 83 µg g −1 (Krishnakumar et al 2020 ) Root 388 µg g −1 Ophiorrhiza rugosa var. decumbens (Gardner ex Thwaites) Deb & Mondal Rubiaceae Whole plant 4.20 µg g −1 (Krishnakumar et al 2020 ) Shoot 2 µg g −1 Root 24 µg g −1 Ophiorrhiza rugosa var. prostrata (D.Don) Deb & Mondal Rubiaceae Stem 0.08% (Gharpure et al 2010 ) Root 0.16% Fruit 0.0165% Young leaves 0.0062% Mature leaves 0.0022% Ophiorrhiza filistipula Miq.…”

Section: Camptothecin: Structure and Its Derivatives/analoguesmentioning

confidence: 99%

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Biotechnological approaches for the production of camptothecin

Banadka,

Narasimha,

Dandin

et al. 2024

Appl Microbiol Biotechnol

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Camptothecin (CPT), an indole alkaloid popular for its anticancer property, is considered the third most promising drug after taxol and famous alkaloids from Vinca for the treatment of cancer in humans. Camptothecin was first identified in Camptotheca acuminata followed by several other plant species and endophytic fungi. Increased harvesting driven by rising global demand is depleting the availability of elite plant genotypes, such as Camptotheca acuminata and Nothapodytes nimmoniana, crucial for producing alkaloids used in treating diseases like cancer. Conservation of these genotypes for the future is imperative. Therefore, research on different plant tissue culture techniques such as cell suspension culture, hairy roots, adventitious root culture, elicitation strategies, and endophytic fungi has been adopted for the production of CPT to meet the increasing demand without affecting the source plant’s existence. Currently, another strategy to increase camptothecin yield by genetic manipulation is underway. The present review discusses the plants and endophytes that are employed for camptothecin production and throws light on the plant tissue culture techniques for the regeneration of plants, callus culture, and selection of cell lines for the highest camptothecin production. The review further explains the simple, accurate, and cost-effective extraction and quantification methods. There is enormous potential for the sustainable production of CPT which could be met by culturing of suitable endophytes or plant cell or organ culture in a bioreactor scale production. Also, different gene editing tools provide opportunities for engineering the biosynthetic pathway of CPT, and the overall CPT production can be improved . Key points • Camptothecin is a naturally occurring alkaloid with potent anticancer properties, primarily known for its ability to inhibit DNA topoisomerase I. • Plants and endophytes offer a potential approach for camptothecin production. • Biotechnology approaches like plant tissue culture techniques enhanced camptothecin production.

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Section: Camptothecin: Structure and Its Derivatives/analoguesmentioning

confidence: 99%

Section: Camptothecin: Structure and Its Derivatives/analoguesmentioning

confidence: 99%

Biotechnological approaches for the production of camptothecin

Banadka,

Narasimha,

Dandin

et al. 2024

Appl Microbiol Biotechnol

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“…Recently, growing attention has been given to CPT production by Ophiorrhiza species in the Rubiaceae family [8]. Ophiorrhiza pumila is one of the most important herbaceous perennial plants.…”

Section: Introductionmentioning

confidence: 99%

Optimal harvest-time to maximize the annual camptothecin production by Ophiorrhiza pumila in a plant factory with artificial light

et al. 2022

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Ophiorrhiza pumila is a medicinal plant that grows in subtropical forests and produces camptothecin (CPT). To determine an optimal harvest time of O. pumila in a plant factory with artificial light (PFAL), we investigated the CPT distribution in each organ and at the developmental stage and estimated the annual CPT production. For this study, the O. pumila plants were grown in controlled environments (16 h light period, photosynthetic photon flux density of 100 μmol m−2 s−1 under white light-emitting diode lamps, air temperature of 28 °C, relative humidity of 80%, and CO2 concentration of 1000 μmol mol−1). First, the stem, root, and seed pod had higher CPT contents than the leaves, flower, and ovary. The optimal harvest time of O. pumila in a PFAL was 63 days after transplanting (DAT), because the CPT content in the whole organs was the highest at the seed-ripening stage. Second, based on these results, the estimated annual CPT production of O. pumila cultivated in a PFAL was 380 mg m−2 y−1 (63 DAT). This value was 4.3 times greater than the annual CPT production by Camptotheca acuminata in a greenhouse. We concluded that the CPT production by O. pumila in a PFAL throughout the year has many advantages, although the demand for electrical energy was high compared to that of Camptotheca acuminata in a greenhouse.

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Recent studies on chemical constituents of Ophiorrhiza plants

Kitajima

2022

J Nat Med

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Ophiorrhiza plants (Family Rubiaceae) are known to produce diverse monoterpenoid indole alkaloids including camptothecin with potent antitumor activity. This review contains a summary of recent chemical studies reported over the past 10 years regarding alkaloids (monoterpenoid indole and tetrahydroisoquinoline alkaloids, and cyclopeptide) in Ophiorrhiza plants. In addition, the alkaloid biosynthetic pathways based on their reported structures were proposed.

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Ophiorrhiza, a promising herbaceous source of the anticancer compound camptothecin

Cited by 6 publications

References 55 publications

Biotechnological approaches for the production of camptothecin

Biotechnological approaches for the production of camptothecin

Optimal harvest-time to maximize the annual camptothecin production by Ophiorrhiza pumila in a plant factory with artificial light

Recent studies on chemical constituents of Ophiorrhiza plants

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