17Study question 18 Can mitochondrial DNA (mtDNA) levels in trophectodermal cells of the blastocyst predict the 19 blastocyst quality, ploidy status, implantation rate and clinical outcomes? 20 Summary answer 21 mtDNA levels in trophectodermal cells of the blastocyst do not associate with the blastocyst 22 quality, ploidy status, implantation potential and clinical outcomes, but can differentiate between 23 aneuploid and euploid blastocysts.24 What we already know 25 mtDNA levels in the trophectodermal cells have been suggested to be associated with blastocyst 26 morphology, ploidy and implantation rates, and has been proposed as biomarker to access 27 blastocyst quality and predict clinical outcomes. However, discrepancies exist if mtDNA levels 28 could serve as a marker for the same.29 Study design and duration 30
A
BSTRACT
Background:
Vitrification of embryos following a single-controlled ovarian stimulation has been the strategy practised now in many
in vitro
fertilisation clinics to minimise the risk of early ovarian hyper stimulation syndrome, to reduce multiple pregnancy rates and to improve cumulative pregnancy rates. In recent years, advances in vitrification techniques and improved culture conditions have led to good post-thaw embryo survival rates, thereby increasing pregnancy rates of frozen embryo transfer (FET) cycles.
Aim:
The aim of this study was to analyse the effect of post-thaw incubation time of frozen embryos on the clinical pregnancy rates (CPRs) of frozen embryo transfer (FET) cycles.
Settings and Design:
This was a retrospective, comparative study done at a teaching hospital in assisted reproductive treatment.
Materials and Methods:
Three hundred and ten FET cycles were analysed, of which 125 had day 2 freezing and 185 had day 3 freezing. Depending upon the day of thawing and day of transfer, FET cycles were divided into six groups: Group 1 (day 2 thawing and day 3 transfer), Group 2 (day 2 thawing and day 4 transfer), Group 3 (day 2 thawing and day 5 transfer), Group 4 (day 3 thawing and day 3 transfer), Group 5 (day 3 thawing and day 4 transfer) and Group 6 (day 3 thawing and day 5 transfer).
Statistical Analysis Used:
Statistical analysis was performed using version 14 R software version 4.0.1 (2020-06-06) (R foundation for Statistical Computing, Vienna, Austria). A
P
< 0.05 is taken as significant.
Results:
The CPR of Group 4 was 42.4% which was more than that of the other groups but it did not reach statistical significance.
Conclusions:
Short incubation time of 2–4 h is as effective as an extended incubation time in terms of CPRs of FET cycles.
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