The emergence and spread of antimicrobial resistance pose a threat to public health globally. Antibiotic-resistant bacteria and genes can disseminate among environments, animals and humans. Therefore, investigation into potential reservoirs of multidrug-resistant bacteria is of great importance to the understanding of putative transmission routes of resistant bacteria and resistance genes. This study aimed to report the occurrence of Escherichia coli harboring the Klebsiella pneumoniae carbapenemase-producing gene (blaKPC) in Psittaciformes rescued from wildlife trafficking in Paraíba State, Brazil. Cloacal swabs were collected from thirty birds and cultured by conventional microbiology using MacConkey and serum tryptone glucose glycerol (STGG) media supplemented with selective antimicrobials. E. coli isolates (n = 43) were identified by phenotypic tests and confirmed by MALDI-TOF. Antimicrobial susceptibility profiles were determined by means of Kirby–Bauer test. All isolates were further screened for extended-spectrum beta-lactamase (ESBL) production, and putative genes encoding ESBL were investigated by PCR. Additionally, blaKPC-harboring strains were genotyped by REP-PCR. A total of 43 E. coli phenotypically resistant isolates were recovered. The highest resistance rate was observed against ciprofloxacin. Among the resistance genes, only blaKPC was found in seven different birds from three species. According to the genotyping, these seven isolates belonged to four different strains. To date, this is the first report on the occurrence of KPC-E. coli in Psittaciformes rescued from trafficking in Northeastern Brazil. Due to the high clinical importance of KPC-E. coli, our findings suggest that wild animals in captivity at wildlife rescue centers can play a role as reservoirs of bacteria that are resistance to Critically Important antimicrobials in human medicine.
As técnicas anatômicas têm sido utilizadas para a preparação de peças para o ensino prático da anatomia, principalmente a macroscópica. Essas técnicas desenvolvidas facilitam o trabalho dos professores durante o ensino e melhoram a compreensão do conteúdo didático. Desta maneira o presente trabalho objetivou desenvolver uma peça anatômica, detalhando o processo de realização da angiotécnica, e apresentando o material como opção de ensino diferenciado das estruturas anatômicas vasculares do rim suíno. A angiotécnica foi realizada em duas etapas: primeiramente a repleção, que consiste no preenchimento de ductos dos vasos sanguíneos dos rins, por meio de injeção de soluções de resina em seu interior. A segunda etapa consistiu na eliminação do tecido orgânico da amostra com ácido HCl, para visibilizar os ductos com resina, essa etapa é chamada de corrosão. O modelo preparado apresentou claramente as artérias, veias e suas ramificações, permitindo a observação de estruturas menores como capilares, mostrando que o órgão renal é bem vascularizado. A técnica demostrou ser uma opção didática válida no ensino do morfológico devido à perfeita observação das estruturas vasculares, permitindo o manuseio prático por parte do corpo docente e discente no ensino da anatomia veterinária.
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