To date, dynamics monitoring oxidative stress on time resolution in vivo has not been achieved due to the limitation of probes, causing the difficulty to comprehensively understand its biological behaviors and functions. Herein, we propose a novel probe, namely highly bright and color tunable "multicenter-emitting" carbon dots (C-dots), for dynamics monitoring oxidative stress in vivo.Combined with experiments and theoretical calculations, we reveal that each C-dot contains multiple conjugated polyaromatic units on their surfaces, which act as fluorescent emitting centers and response sites of hypochlorous acid (HClO) to achieve an instantaneously color-change readout (107 nm, from yellow-green to blue). Accordingly, the proposed C-dots enable the observation of HClO burst and dynamics evolution at fresh wounds of zebrafish larvae. Such a burst occurs only at 1−5 s after cutting, which is an unprecedented thought regarding oxidative stress dynamics. Furthermore, the present study indicates that HClO molecules are principally generated in situ rather than migration.
We herein present a reactive site regulation strategy for fabricating well-defined Janus and core@shell gold nanorod-Cu2−xS dual metal–semiconductor plasmonic supraparticles.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.