Cell cultures are indispensable for both basic and applied research. Advancements in cell culture and analysis increase their utility for basic research and translational applications. A marked development in this direction is advent of three-dimensional (3D) cultures. The extent of advancement in 3D cell culture methods over the past decade has warranted referring to a single cell type being cultured as an aggregate or spheroid using simple scaffolds as "traditional." In recent years, the development of "next-generation" devices has enabled cultured cells to mimic their natural environments much better than the traditional 3D culture systems. Automated platforms like chip-based devices, magnetic-and acoustics-based assembly devices, dielectrophoresis (DEP), micro pocket cultures (MPoC), and 3D bio-printing provide a dynamic environment compared to the rather static conditions of the traditional simple scaffold-based 3D cultures. Chip-based technologies, which are centered on prin-

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Objectives: Three-dimensional (3D) printing has gained significance for human health-care applications in recent years. Some of these applications include obtaining models which mimic anatomical parts. One other parallel development in the biological research area is the development of 3D cell cultures. Such cultures are now becoming the material of choice for in vitro experiments, fast replacing the traditional adherent/monolayer 2D culture approaches. We present here, a method to obtain 3D prints of 3D aggregates of three human cancer cell lines. Such 3D prints can be useful models to understand solid tumor morphologies and also as effective teaching models. Materials and Methods: Photomicrographs of the 3D aggregates of the human cancer cell lines SiHa, MCF-7, and A549 (human cervical cancer, breast cancer, and non-small cell lung cancer cell lines, respectively) were obtained using inverted phase contrast microscopy. Conversion of normal jpeg images into 3D files was performed using the lithophane method and CAD files obtained. The CAD files thus generated were used to print the objects using the Stratasys Polyjet J750 3D Printer. Results: We could obtain 3D prints of SiHa, MCF-7, and A549 (human cervical cancer, breast cancer, and non-small cell lung cancer cell lines, respectively) 3D aggregates/spheroids. Conclusion: It is hoped that this approach will be useful for studying solid tumor morphologies in finer details. Furthermore, other benefits of such 3D prints would be in them being excellent models for teaching purposes.

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