Dynamic Three-Dimensional Cell-Culture Systems for Enhanced &lt;i&gt;in Vitro&lt;/i&gt; Applications

Aishwarya, Pargaonkar; Agrawal, Gatika; Sally, Jennifer; Ravi, Maddaly

doi:10.18520/cs/v122/i2/149-160

Cited by 4 publications

(3 citation statements)

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“…Dynamic 3D culture systems in comparison with static culture systems can improve nutrient supply and other relevant physio-chemical factors. In addition, dynamic 3D culture improves cell–cell and cell–ECM interactions, proliferating, and differentiation, by higher cell–cell adhesion and cell-ECM remodeling [ 95 ]. Kang et al have shown that the bioreactor EV yield is nearly seven times more EVs per cell in 24 h compared to static conditions by designing a flat-plate bioreactor [ 96 ].…”

Section: Limitations In Vitro Cartilage Research and Co-culture Engin...mentioning

confidence: 99%

Co-culture engineering: a promising strategy for production of engineered extracellular vesicle for osteoarthritis treatment

Esmaeili,

Hosseini,

Baghaban Eslaminejad

2024

Cell Commun Signal

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The therapeutic effects of extracellular vesicles (EVs) have been identified as a significant factor in intercellular communication in different disease treatments, including osteoarthritis (OA). Compared to the conventional approaches in treating OA, EV therapy is a non-invasive and cell-free method. However, improving the yield of EVs and their therapeutic effects are the main challenges for clinical applications. In this regard, researchers are using the EV engineering potential to overcome these challenges. New findings suggest that the co-culture strategy as an indirect EV engineering method efficiently increases EV production and quality. The co-culture of mesenchymal stem cells (MSCs) and chondrocytes has improved their chondrogenesis, anti-inflammatory effects, and regenerative properties which are mediated by EVs. Hence, co-culture engineering by considerable systems could be useful in producing engineered EVs for different therapeutic applications. Here, we review various co-culture approaches, including diverse direct and indirect, 2D and 3D cell cultures, as well as static and dynamic systems. Meanwhile, we suggest and discuss the advantages of combined strategies to achieve engineered EVs for OA treatment.

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Section: Limitations In Vitro Cartilage Research and Co-culture Engin...mentioning

confidence: 99%

Co-culture engineering: a promising strategy for production of engineered extracellular vesicle for osteoarthritis treatment

Esmaeili,

Hosseini,

Baghaban Eslaminejad

2024

Cell Commun Signal

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“…Recent advancements in label-free cell culturing techniques have also popularized the use of acoustic-based and di-electrophoresis-based systems to culture cells in three dimensions without using scaffolds. Acoustic-based assembly employs non-invasive sound waves to assemble suspended cells in a culture medium, aggregating them to form spheroids [ 52 ]. Acoustic-based or acoustofluidic assembly is a contact-free and label-free approach utilizing bulk acoustic waves and Voronoi tessellation to cluster suspended cells; then, acoustic streaming is used to aggregate them into spheroids [ 53 ].…”

Section: Three-dimensional Cell Culturesmentioning

confidence: 99%

“…Acoustofluidic 3D cell culture systems integrate acoustic waves within microfluidic or disposable capillaries or devices to rapidly aggregate cells, forming uniform spheroids within minutes [ 53 ]. The di-electrophoresis-based system is another label-free technique that polarizes suspended cells in the culture medium through a non-uniform electric field, forming electric dipoles [ 52 ]. The cells are separated by a repulsive force, and the force subjected on the suspended cells is dependent on the cell size, allowing cells of identical sizes to aggregate together to form spheroids [ 54 ].…”

Section: Three-dimensional Cell Culturesmentioning

confidence: 99%

Vascularization Strategies in 3D Cell Culture Models: From Scaffold-Free Models to 3D Bioprinting

Anthon

Valente

2022

IJMS

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The discrepancies between the findings in preclinical studies, and in vivo testing and clinical trials have resulted in the gradual decline in drug approval rates over the past decades. Conventional in vitro drug screening platforms employ two-dimensional (2D) cell culture models, which demonstrate inaccurate drug responses by failing to capture the three-dimensional (3D) tissue microenvironment in vivo. Recent advancements in the field of tissue engineering have made possible the creation of 3D cell culture systems that can accurately recapitulate the cell–cell and cell–extracellular matrix interactions, as well as replicate the intricate microarchitectures observed in native tissues. However, the lack of a perfusion system in 3D cell cultures hinders the establishment of the models as potential drug screening platforms. Over the years, multiple techniques have successfully demonstrated vascularization in 3D cell cultures, simulating in vivo-like drug interactions, proposing the use of 3D systems as drug screening platforms to eliminate the deviations between preclinical and in vivo testing. In this review, the basic principles of 3D cell culture systems are briefly introduced, and current research demonstrating the development of vascularization in 3D cell cultures is discussed, with a particular focus on the potential of these models as the future of drug screening platforms.

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Portable dielectrophoresis for biology: ADEPT facilitates cell trapping, separation, and interactions

Julius,

Akgül,

Krishnan

et al. 2024

Microsyst Nanoeng

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Dielectrophoresis is a powerful and well-established technique that allows label-free, non-invasive manipulation of cells and particles by leveraging their electrical properties. The practical implementation of the associated electronics and user interface in a biology laboratory, however, requires an engineering background, thus hindering the broader adoption of the technique. In order to address these challenges and to bridge the gap between biologists and the engineering skills required for the implementation of DEP platforms, we report here a custom-built, compact, universal electronic platform termed ADEPT (adaptable dielectrophoresis embedded platform tool) for use with a simple microfluidic chip containing six microelectrodes. The versatility of the open-source platform is ensured by a custom-developed graphical user interface that permits simple reconfiguration of the control signals to address a wide-range of specific applications: (i) precision positioning of the single bacterium/cell/particle in the micrometer range; (ii) viability-based separation by achieving a 94% efficiency in separating live and dead yeast; (iii) phenotype-based separation by achieving a 96% efficiency in separating yeast and Bacillus subtilis; (iv) cell–cell interactions by steering a phagocytosis process where a granulocyte engulfs E. coli RGB-S bacterium. Together, the set of experiments and the platform form a complete basis for a wide range of possible applications addressing various biological questions exploiting the plug-and-play design and the intuitive GUI of ADEPT.

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Dynamic Three-Dimensional Cell-Culture Systems for Enhanced <i>in Vitro</i> Applications

Cited by 4 publications

References 0 publications

Co-culture engineering: a promising strategy for production of engineered extracellular vesicle for osteoarthritis treatment

Co-culture engineering: a promising strategy for production of engineered extracellular vesicle for osteoarthritis treatment

Vascularization Strategies in 3D Cell Culture Models: From Scaffold-Free Models to 3D Bioprinting

Portable dielectrophoresis for biology: ADEPT facilitates cell trapping, separation, and interactions

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