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Good-quality single crystals of the nonlinear optical (NLO) crystal l-histidinium-4-nitrophenolate 4-nitrophenol (LHPP) with a large size of 40 × 25 × 10 mm3 have been grown by the slow cooling method. Its morphology has been indexed to reveal that the crystal is a rhombohedra with major forms of (−100) and (−101). The optical transmittance range has been identified by UV–vis NIR studies. Second-harmonic generation (SHG) on powder samples has been measured using the Kurtz and Perry technique, and the results display that LHPP is a phase-matchable NLO material with its SHG efficiency being 3.55 times as large as that of KDP. Its specific heat and the principal thermal expansion along the principal crystal axes have been measured at different temperatures. The average principal thermal expansion coefficients have been calculated on the basis of experimental measurements between 298 and 410.5 K, and the relationship between the structure and the thermal properties has been discussed. Surface morphologies of the laser-induced damaged crystal were observed with an optical microscope, and the nature origin of the damage was analyzed.

BackgroundXylanase is one of the most extensively used biocatalysts for biomass degradation. However, its low catalytic efficiency and poor thermostability limit its applications. Therefore, improving the properties of xylanases to enable synergistic degradation of lignocellulosic biomass with cellulase is of considerable significance in the field of bioenergy.ResultsUsing fragment replacement, we improved the catalytic performance and thermostability of a GH10 xylanase, XylE. Of the ten hybrid enzymes obtained, seven showed xylanase activity. Substitution of fragments, M3, M6, M9, and their combinations enhanced the catalytic efficiency (by 2.4- to fourfold) as well as the specific activity (by 1.2- to 3.3-fold) of XylE. The hybrids, XylE-M3, XylE-M3/M6, XylE-M3/M9, and XylE-M3/M6/M9, showed enhanced thermostability, as observed by the increase in the T50 (3–4.7 °C) and Tm (1.1–4.7 °C), and extended t1/2 (by 1.8–2.3 h). In addition, the synergistic effect of the mutant xylanase and cellulase on the degradation of mulberry bark showed that treatment with both XylE-M3/M6 and cellulase exhibited the highest synergistic effect. In this case, the degree of synergy reached 1.3, and the reducing sugar production and dry matter reduction increased by 148% and 185%, respectively, compared to treatment with only cellulase.ConclusionsThis study provides a successful strategy to improve the catalytic properties and thermostability of enzymes. We identified several xylanase candidates for applications in bioenergy and biorefinery. Synergistic degradation experiments elucidated a possible mechanism of cellulase inhibition by xylan and xylo-oligomers.

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We demonstrate an enhancement mechanism and thermal model for intra-cavity pumped lasers, where resonance enhancement in intra-cavity pumped Ho laser was achieved by manipulating the wavelength-drift nature of the Tm laser for the first time. Optical conversion efficiency of 37.5% from an absorbed 785 nm diode laser to a Ho laser was obtained with a maximum output power of 7.51 W at 2122 nm, which is comparable to the conversion efficiency in 1.9 μm LD pumped Ho lasers. Meanwhile, more severe thermal effects in the Ho-doped gain medium than the Tm-doped one at high power operation were verified based on the built thermal model. This work benefits the design or evaluation of intra-cavity pumped lasers, and the resonance enhancement originated from the difference in reabsorption loss between stark levels at the lasing manifolds of quasi-three-level rare-earth ions has great interest to improve the existing intra-cavity pumped lasers or explore novel lasers.

We explored Q-switching mechanism for the newly proposed Tm/Ho composite laser via developing a hybrid resonator for separating the intra-cavity Tm laser modulated by the saturable absorber (SA). With a Cr:ZnSe SA, successful passively Q-switching process with the maximum average output power of 474 mW and the shortest pulse width of 145 ns were obtained at the pulse repetition frequency of 7.14 kHz, where dual wavelength oscillation in both 2090 nm and 2097 nm was observed. This work provides an effective way for a direct laser diode (LD) pumped Q-switched Ho laser, which is compact and accessible. Furthermore, the current SA could be replaced by the 2D materials with broadband saturable absorption such as topological insulators or transition-metal dichalcogenides for seeking novel PQS lasers.