| There is an increase population growth in Indonesia. Therefore, it is necessary to control this through the use of antifertility agents. Both cepoka eggplant (Solanum torvum S.) and Kapok seeds (Ceiba pentandra G.) have a role as antifertility agents as contains solasodine and gossypol compounds respectively. This study aims to determine the difference between the effect of cepoka eggplant extract and Kapok seed as male antifertility agents on the expression of p53 protein in the seminiferous tubular tissue and the number of Leydig cells in rats. A total of 18 male Wistar rats, 2.5-3 months old and 190-320 g weight were randomly divided into 3 groups with 6 replicats, as follows: the negative control group (K-) without treatment, group 1 (G1) given 1 g/kg Cepoka eggplant extract, and group 2 (G2) admistered 0.1 g/kg Kapok seed extract. The results showed that the administration of 0.1 g/kg Kapok seed extract (Ceiba pentandra G.) significantly increased the expression of p53 protein and reduced the number of Leydig cells compared with the administration of 1 g/kg Cepoka eggplant extract (Solanum torvum S.). It was concluded that Kapok seed extract can be used as antifertility agent as increase the expressions of p53 protein and reduce the number of Leydig cells.Citation | Hendrawan VF, Cakrawati LS, Aulanniam A, Wulansari D, Oktanella Y, Agustina GC (2019). Impact of cepoka eggplant extract (solanum torvum s.) And kapok seed (ceiba pentandra g.) On expression of p53 protein and the number of leydig cells in rats. Adv. Anim. Vet. Sci. 7(9): 732-737.
Sapi merupakan ternak ruminansia yang sangat potensial di Indonesia. Setiap induk ternak mempunyai tiga kemungkinan status reproduksi yaitu: berada pada kondisi kesuburan yang normal, kondisi kemajiran yang ringan atau infertil, kondisi kemajiran yang tetap atau steril. Infertilitas adalah keadaan dimana derajat kesuburan ternak menurun yang disebabkan oleh adanya gangguan organ reproduksi. Infertil sifatnya sementara dan masih dapat diobati dan bila pengobatan berhasil maka masih dapat bereproduksi kembali. Kemajiran dengan derajat yang berat sifatnya permanen atau steril yaitu berhentinya proses reproduksi secara penuh dan tidak dapat diobati. Banyak faktor yang dapat menyebabkan kemajiran pada ternak khususnya sapi, yang mayoritas merupakan gangguan hormonal terutama hormon reproduksi yang menimbulkan gejala seperti silent heat (birahi tenang) dan subestrus (birahi pendek) disebabkan oleh rendahnya kadar hormon estrogen. Sedangkan untuk kasus delayed ovulasi (ovulasi tertunda), anovulasi (kegagalan ovulasi) dan kista folikuler disebabkan oleh rendahnya kadar hormon gonadotropin (FSH dan LH). Gangguan hormonal tersebut dapat terjadi pada indukan sapi perah berproduksi susu tinggi serta akibat adanya endometritis yang berasal dari kelahiran yang tidak normal, seperti: abortus, retensi sekundinarum, kelahiran premature, kelahiran kembar, distokia, ataupun perlukaan saat melakukan pertolongan kelahiran. Tujuan dari kegiatan ini adalah untuk menanggulangi gangguan reproduksi sapi serta dapat memperbaiki performa reproduksi sapi dengan melihat peningkatan angka kejadian birahi pada kelompok ternak di Desa Babakan Kecamatan Karangploso Kabupaten Malang.
The presence of the solasodine and gossypol compounds in Kapok seeds (Ceiba pentandra) and cepoka eggplant (Solanum torvum) suggest the use of these two as an herbal contraceptive ingredient in male animal fertility. This study aimed to determine the effect of cepoka eggplant extract (S. torvum) and kapok seed (C. pentandra) on infertility in rats (Rattus norvegicus) through NFkB expression and seminiferous tubule diameter. The study was carried out on 75 to 90 days old male Wistar rats having 150-200 grams of body weight. The experimental design used in this study was a completely randomized design (CRD) with three treatment groups i.e. C (control), KP1, and KP2 having six rats in each group. Here the rats of group C were not treated with gossypol or solasodine while the rats of group KP1 were treated with solasodine compound at a dose of 1g/kg BW and rats of group KP2 were treated with gossypol induction at a dose of 0.1 g/kg BW. Kapok seed extract and eggplant cepoka extract were extracted by the maceration method with 70% ethanol solvent. NFkB expression was examined using the immunohistochemical (IHK) method, which was analyzed with immunoratio software. Histopathological preparations (HE) of seminiferous tubule diameter were analyzed using raster image software 3. All the obtained data were analyzed by the One Way ANOVA test and Tukey's follow-up test with a 95% confidence level (α = 0.05). The results showed that the administration of cepoka eggplant extract could significantly increase the expression of NFkB (p<0.05) with an average amount of 87.22 ± 6.89 with a dose of 1 g/kg BW. Treatment with Kapok seed extract can reduce the diameter of the seminiferous tubules (STs) by an average of 0.31 ± 0.016 at a dose of 0.1 g/kg BW. The results of the study can be concluded that the extract of cepoka eggplant and kapok seeds can be used as candidates for herbal contraceptives.
This study aimed to determine the effect of black cumin extract (Nigella sativa) on the histopathological of the liver and kidneys in albino rats exposed to organophosphates. This study used 20 male mice divided into five treatment groups, i.e. (K-) without organophosphate and black cumin, (K+) induced by organophosphate 40 mg/kg BW, treatment group induced by organophosphate 40 mg/kg BW and black cumin extract as therapy (P1 ) 200 mg/kg BW, (P2) 400 mg/kg BW, and (P3) 600 mg/kg BW. Organophosphate administration for eight weeks and black seed extract therapy was given for three weeks then the mice were euthanized, the liver and kidney organs were taken for histopathological observation using Haematoxylin-Eosin (HE). Data were analyzed using OneWay ANOVA test followed by Tukey test. The results showed that there were significant differences between the treatment groups for liver and kidney damage (p<0,05). Hepatic degeneration and necrosis in the P2 and P3 groups differed from K+ with an average of 2,24±0,9, in the kidneys with an average of 2,52±0,8. It can be concluded that black cumin extract has a significant effect on improving the liver and kidneys in groups P3 (600 mg/kgBW).
Diazinon is an organophosphate pesticide that is often used in agricultural business sectors. Nevertheless, this type of pesticide could negatively inhibit the acetylcholinesterase, resulting cell apoptosis and hormonal disruption of the reproductive system. The major of this study was to evaluate the effectiveness of black cumin seeds as antioxidants on the expression of caspase-3 and sertoli cells in spermatogenesis. A total of 20 male mouses were divided into five groups, K-(negative control, no treatment), K+ (positive control, 40 mg diazinon/kg BW); P1 (40 mg diazinon/kg BW + 200 mg black cumin/kg BW); P2 (40 mg diazinon/kg BW + 400 mg black cumin/kg BW); P3 (40 mg diazinon/kg BW + 600 mg black cumin/kg BW) throughout trials. The results showed that the black cumin seeds powder significantly decreased the caspase-3 expression, whereby P3 revealed the lower value compared to other treatment groups (P < 0.05). Besides, an increased the number of Sertoli cells was significantly in line the supplementation of black cumin powder (P < 0.05), showing P3 had stronger effect than their counterpars. Furthermore, this study suggests that the administration of black cumin seed powder could decrease the expression of caspase-3 and increase the number of Sertoli cells in mouses subjected diazinon.
Rabies related to increasing canine population in Bali. Uncontrolled wild animal populations caused disease transmission from animal to human. Various attempt at population control are carried out such as the use of natural contraception. Some compounds are known to have potential as antifertility are solasodine and gosipol. Solasodine is known have an antifertility affect. Gosipol, fenolic compound in Ceiba pentandra, inhibits spermatogenesis, reduce sperm concentration, motility and viability. This research aims to compare effectiveness of terong cepoka and Ceiba pentandra as antifertility. This research was conducted at Mei-November 2018. Eight-teen rats were used in this study and divided into three groups: control, P1 extract Solanum torvum 1g/kg BW and P2 extract Ceiba pentandra 0,1g/kg BW PO. Rats were treated with extract for 10 days and euthanazed at day 11. Testis were collected for histopathology using HE staining to observe spermatogenesis and using immunohistochemistry to observe LH expression. The result are analyzed using one way ANOVA P<0.05. the result show that extract solanum torvum 1g/kg BW and ceiba pentandra 0,1g/kgBW cannot reduce spermatogenesis and LH expression. This study used crude extract which still consist any other compound like antioxidant. Future study we need use isolated and pure solasodine and gosipol.
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