Background and aims
Persimmon (Diospyros kaki) is an economically
important fruit tree species with complex flowering characteristics. To obtain
accurate expression pattern analysis results, it is vital to select a reliable gene
for the normalization of real-time quantitative polymerase chain reaction data. The
aim of this study was to identify the optimal internal control gene among six
candidate genes for gene expression analysis in different persimmon organs and
developmental stages.
Materials and methods
This analysis was conducted using geNorm and NormFinder software to show
differences in the stability of the six reference genes among tissues and floral
developmental stages of the same plant.
Results
Although genes that exhibited moderate expression in NormFinder revealed
slightly different expression stabilities than those obtained by geNorm, both sets of
results showed that GAPDH was the best reference gene in
different organs and floral buds at different developmental stages, whereas
18SrRNA was the least stable gene.
Conclusions
Based on the overall ranking, GAPDH is the most
suitable reference gene and is highly recommended for gene expression studies in
different organs and different developmental stages of persimmon. This study provides
useful reference data for future gene expression studies and will contribute to
improving the accuracy of gene expression results in persimmon.
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