Transcriptome sequencing and comparative analysis between male and female floral buds of the persimmon (Diospyros kaki Thunb.)

Li, Shuzhan; Sun, Peng; Du, Gaigai; Wang, Liyuan; Li, Huawei; Fu, Jianmin; Suo, Yujing; Han, Weijuan; Mai, Yini; Li, Fangdong

doi:10.1016/j.scienta.2018.11.073

Cited by 15 publications

(10 citation statements)

References 41 publications

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“…As a result, we can distinguish flower sexuality using the OGI/MeGI system served as an early sex diagnosis technology for future investigations in persimmon (Kajita et al, 2015;Zhang et al, 2016). Moreover, our previous study showed that high levels genes homologous to IAA32, beta-amyrin 28-oxidase-like, and GA20OX2 may stimulate the development of male floral buds, while the genes homologous to MeGI and ACO showed female promoting effects (Li et al, 2019). Therefore, it is necessary to detect optimal internal control genes for male and female buds separately for data analysis accurately.…”

Section: Discussionmentioning

confidence: 98%

Selection and validation of reference genes for quantitative gene expression analyses in persimmon (Diospyros kaki Thunb.) using real-time quantitative PCR

Wang

et al. 2019

BioFut

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Background and aims Persimmon (Diospyros kaki) is an economically important fruit tree species with complex flowering characteristics. To obtain accurate expression pattern analysis results, it is vital to select a reliable gene for the normalization of real-time quantitative polymerase chain reaction data. The aim of this study was to identify the optimal internal control gene among six candidate genes for gene expression analysis in different persimmon organs and developmental stages. Materials and methods This analysis was conducted using geNorm and NormFinder software to show differences in the stability of the six reference genes among tissues and floral developmental stages of the same plant. Results Although genes that exhibited moderate expression in NormFinder revealed slightly different expression stabilities than those obtained by geNorm, both sets of results showed that GAPDH was the best reference gene in different organs and floral buds at different developmental stages, whereas 18SrRNA was the least stable gene. Conclusions Based on the overall ranking, GAPDH is the most suitable reference gene and is highly recommended for gene expression studies in different organs and different developmental stages of persimmon. This study provides useful reference data for future gene expression studies and will contribute to improving the accuracy of gene expression results in persimmon.

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Section: Discussionmentioning

confidence: 98%

Selection and validation of reference genes for quantitative gene expression analyses in persimmon (Diospyros kaki Thunb.) using real-time quantitative PCR

Wang

et al. 2019

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“…There is also evidence that CKs are involved in the male reproductive development of Arabidopsis thaliana ( Kinoshita-Tsujimura and Kakimoto, 2011 ). Furthermore, high levels of ZT could promote the differentiation of male floral buds leading to female unisexual flowers in D. kaki by enhancing the development of pistil primordia ( Sun et al, 2017 ; Li S. et al, 2019 ). Cytokinin oxidase/dehydrogenase (CKXs) catalyzes the irreversible degradation of CKs.…”

Section: Discussionmentioning

confidence: 99%

Integrative Analyses of Transcriptomics and Metabolomics in Sex Differentiation of Mulberry Flowers

Liu

Zhu

et al. 2022

Front. Mol. Biosci.

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Sex determination and sex differentiation of plants are important physiological processes of plant development. Mulberry (Morus indica L.) is an important economic tree being cultivated in sericulture countries, and mulberry leaf is commonly used for sericulture. The transcriptomic and metabolomic differences between the staminate flowers (SFs) and pistillate flowers (PFs) of mulberry were investigated by RNA sequencing and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Overall, we uncovered 4,230 genes and 209 metabolites are significantly differentially expressed between the SFs and PFs of mulberry. The combined transcriptomic and metabolomic analysis revealed these differentially expressed genes (DEGs) and differentially expressed metabolites (DEMs) are involved in flavonoid biosynthesis, galactose metabolism, plant–pathogen interaction, and starch and sucrose metabolism, and these detected DEGs and DEMs may be associated with sex differentiation of mulberry through the regulation of the enrichment pathways, such as the MAPK pathway, flavonoid biosynthesis, galactose metabolism, plant–pathogen interaction, and starch and sucrose metabolism. This study will provide a rich source for the analysis of the molecular mechanism of mulberry sex differentiation processes.

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“…Intensive studies of the regulation mechanisms of sex expression will provide important insights into the genetic regulation of G. sinensis and are useful for genetic studies and breeding applications. Particularly, the transcriptome sequencing strategy has been applied to detect differentially expressed genes of different sex types, such as those in Salix suchowensis [ 25 ], Asparagus officinalis [ 26 ], Diospyros kaki [ 27 ], and so on. We are now using this method to detect the sex-biased expression genes, and further analysis will be expected to add new insights of the genetic regulation of G. sinensis sex expression.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide analysis of microsatellite and sex-linked marker identification in Gleditsia sinensis

2020

BMC Plant Biol

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Background Gleditsia sinensis Lam. (Leguminosae), a dioecious perennial arbor, demonstrates important medicinal properties and economic value. These properties can be harnessed depending on the sex of the plant. However, the sex of the plants is difficult to identify accurately through morphological methods before the flowering. Results We used bulked segregant analysis to screen sex-specific simple sequence repeat (SSR) markers in G. sinensis . Five male and five female plants were pooled to form the male and female bulks, respectively, and subjected to whole-genome sequencing. After high-throughput sequencing, 5,350,359 sequences were obtained, in which 2,065,210 SSRs were searched. Among them, the number of duplicated SSRs was the highest. The male plants could reach 857,874, which accounted for 60.86% of the total number of male plants. The female plants could reach 1,447,603, which accounted for 56.25% of the total model of the female plants. Among all the nucleotide repeat types, the A/T-rich motif was the most abundant. A total of 309,516 female strain-specific SSRs were selected by clustering. After designing the primers, the male and female gene pools were amplified, and five pairs of primers (i.e., 27, 34, 36, 39, and 41) were found to amplify the differential bands in the male and female gene pools. Using the five pairs of primers, we performed PCR verification on 10 individuals of known sex, which constructed the gene pool. The female plants amplified a single fragment of lengths (i.e., 186, 305, 266, 203, and 260 bp) and no male plant strip, thereby completing the identification of the male and female sexes of the G. sinensis. Conclusions This study provides accurate sex identification strategies between female and male plants, thus improving the utilization rate of G. sinensis resources.

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Transcriptome sequencing and comparative analysis between male and female floral buds of the persimmon (Diospyros kaki Thunb.)

Cited by 15 publications

References 41 publications

Selection and validation of reference genes for quantitative gene expression analyses in persimmon (Diospyros kaki Thunb.) using real-time quantitative PCR

Selection and validation of reference genes for quantitative gene expression analyses in persimmon (Diospyros kaki Thunb.) using real-time quantitative PCR

Integrative Analyses of Transcriptomics and Metabolomics in Sex Differentiation of Mulberry Flowers

Genome-wide analysis of microsatellite and sex-linked marker identification in Gleditsia sinensis

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