Poincianella pyramidalis (catingueira) is a toxic plant associated with abortions, embryonic losses and malformations in goats and laboratory animals. Outbreaks and sporadic cases of spontaneous intoxication in sheep have been reported in the last five years in the agreste of Pernambuco. Due to the occurrence of these spontaneous cases and the lack of researches in sheep, an experimental study was carried out to evaluate the teratogenic effects of P. pyramidalis on sheep. In the experiment, 16 female and three male sheeps, mixed breed, were used. Females were divided in four groups, with four animals per group and maintained in individual stalls, underwent estrus synchronization and mating by natural mating and after 30 days the diagnosis was made to confirm gestation. Groups were named group 1 (G1), group 2 (G2), group 3 (G3), and group 4 (G4). In G1 (control) the ewes were fed with 1% of concentrate supplementation (CS) based on live weight (LW) and 2% of maize silage (MS) with a daily intake of 3% dry matter (DM) based on LW. In G2 feeding was constituted of 1% of CS based on LW and 2% of forage, including 2% of roughage, 50% was hay of P. pyramidalis and the other 50% of DM. In G3 feed was constituted of 1% of CS based on LW and 2% of roughage. Among these 2% of roughage, 80% was hay of P. pyramidalis and the other 20% of DM. In G4 the sheep were fed with 1% CS based on the LW and the roughage consisting of 100% P. pyramidalis hay.
Background:The Gurltia paralysans nematode was initially described in Chile and for many years it was believed that the disease caused by this parasite was restricted to this country. However, in Argentina, Uruguay and more recently in Brazil, among other countries, cases of Gurltiosis have been described in both domestic and wild cats. This disease is chronic and debilitating due to the progressive paralysis developed. This study aimed to describe the clinical, epidemiological and pathological aspects of G. paralysans infection in domestic cats of the Agreste region of the state of Pernambuco, Northeast Brazil. Case: Clinical, epidemiological and pathological aspects of G. paralysans infection in domestic cats in the rural area of two Agreste municipalities in the state of Pernambuco, Brazil, are described. Seven farms were visited, in which 11 male and female affected felines were evaluated. Among these, euthanasia was performed in four cases, at the owners' request and due to the advanced stage of the disease. Clinical signs began with ataxia of the pelvic limbs and evolved to jumping difficulty, lateral falls, muscle atrophy, pelvic limb scarring, and paralysis at the most severe stage of the disease, which developed in a one-year period, approximately. According to the owners, the affected cats died between six months and one year after the initial clinical signs. At necropsy, there were segments of the spinal cord with extensive reddish areas in the dura, between T7 and S2, corresponding to varices. These were characterized by numerous congestive, dilated and tortuous blood vessels observed in the dorsal plane, but more pronounced in the ventral plane of the meninges. In the bladder, multifocal areas of hemorrhage were observed. Histologically, vascular lesions in veins and venules of the leptomeninges were characterized by venous varices with thrombosis, fibrosis and intravascular parasites associated with moderate, chronic non-suppurative meningoencephalomyelitis and Wallerian degeneration. Macroscopic and microscopic lesions were more pronounced in the lumbar region of the spinal cord. Discrete lesions similar to those found in the spinal cord were observed in the encephalon. Feline gurltiosis was confirmed by epidemiological, clinical and spinal cord lesions, associated with the presence of intravascular parasites in veins and venules of the leptomeninges. The identification of Gurltia paralysans was concluded due to the characteristic morphology of the parasite in the vessels. Discussion: The diagnosis of Gurltiosis can be performed by clinical-epidemiological findings and histopathological evaluation of the nervous system demonstrating the parasite inside the vessels. The parasite has affinity for the nervous tissue, especially the lumbar spinal cord, in which it can be found in the nervous parenchyma and not only in the veins as previously believed. The chronicity of the disease can be affirmed by clinical signs, secondary lesions, such as cystitis, and the evolution of the condition. The stasis of b...
O objetivo desse estudo foi avaliar os efeitos da laserterapia na lesão do tendão calcanearcomum de ratos Wistar. Foram utilizados 12 ratos machos adultos, distribuídosaleatoriamente em dois grupos (L=laser e C=controle). Todos foram submetidos àtendinopatia unilateral (escolha aleatória) mediante compressão transversal do tendão (10segundos) com pinça Halstead mosquito, assim como escarificações (com bisturi). Após 24horas da indução da lesão os animais do grupo L receberam laser (904 nm/3 J/cm²/9s) por 20dias. Os do grupo C foram manipulados como se fossem receber a radiação. Após 3, 7, 14 e21 dias da realização da lesão, três ratos de cada grupo foram submetidos à eutanásia, e ostendões obtidos para análise histomorfométrica. As amostras foram processadas como derotina e os fragmentos corados com hematoxilina-eosina, tricrômico de Masson e PicrosiriusRed. Os dados foram analisados por ANOVA, a 5% de probabilidade e análise de regressão.Não houve diferença entre grupos nem entre tempos para as características hemorragia,angiogênese, espessamento do epitendão. Independente do tratamento ocorreu diminuição(p=0,0129) da formação de aderência fibrinosa (do 3o ao 21o dias). Por outro lado, a avaliaçãomorfométrica revelou maiores (p=0,0120) quantidades de fibroblastos no grupo que recebeulaserterapia, não havendo efeito de tempo. Avaliação semiquantitativa, revelou maiores(p=0,0000) quantidades de fibroblastos no grupo tratado, porém nessa análise, a quantidadedessas células aumentou com o tempo (p=0,0001) em ambos os grupos. Diferentemente,ANOVA revelou redução do infiltrado inflamatório do 3o ao 21o dia em ambos os grupos(histologia: p=0,0003; morfometria: p=0,0000). Embora não tenha ocorrido diferença entregrupos na quantidade de fibras colágenas (I e III), a morfometria revelou que os ratos dogrupo L apresentaram maiores (p=0,0096) quantidades de fibras colágenas do que do grupoC. Para essa característica, não ocorreu efeito do tempo, ainda que tenha sido observada maior(p=0,0000) organização da deposição de colágeno (7o dia). A quantidade de fibras colágenasdeterminada por histologia revelou efeito apenas do tempo (p=0,0005), independente dotratamento, ocorrendo aumento dessa variável até o 14o dia, com posterior redução. Alaserterapia iniciada 24 horas após indução cirúrgica de lesão no tendão calcanear de ratosWistar apresenta a vantagem de aumentar em qualidade e quantidade as fibras colágenas,assim como os fibroblastos, células fundamentais na síntese dos colágenos.
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