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Summary Gossypol acetic acid at the dose of 5 mg/rat/day for 2 and 4 weeks did not cause any significant effect on the body weight, testis, epididymis, seminal vesicle and prostate weight, nor gossypol treatment had any significant effect on the activities of acid phosphatase and succinic dehydrogenase in the testis. Changes in the testis ATPase activity were, however, significant after gossypol treatment. During the course of present investigations no effect of gossypol treatment on 3H thymidine incorporation into DNA of testicular cells was observed, nor there were any changes in the DNA and total protein content of the testis after gossypol treatment. Gossypol treatment did not cause any effect on the plasma Na+ level. However, transient decrease in the plasma K+ level was observed; decrease in K+ level two weeks after gossypol treatment was restored to normal after 4 weeks of gossypol treatment. No changes in the histology of the testis were observed 2 weeks after gossypol treatment but marked inhibition of spermatogenesis was observed 4 weeks after gossypol treatment. Motility of vas deferens spermatozoa was also markedly inhibited 4 weeks after gossypol treatment. In the light of the present observations and those of others, there is a clear demonstration that gossypol acts directly on the spermatozoa and on the testis; at both the sites the drug interferes in the ATPase activity. Untersuchungen über die Antifertilitätssubstanz Gossypol‐Essigsäure. III. Der Einfluß von Gossypol‐Essigsäure auf den Rattenhoden Zusammenfassung Gossypol‐Essigsäure (G) ‐ in einer Dosis von 5 mg/Ratte/Tag für 2 und 4 Wochen appliziert ‐ verursacht keine signifikanten Veränderungen des Körpergewichtes, des Gewichtes der Hoden, Nebenhoden, Samenblasen und Prostata; G‐Behandlung hat auch keinen Einfluß auf die Aktivität der sauren Phosphatase und der Succinatdehydrogenase des Hodens. Demgegenüber konnte eine Änderung der ATPase‐Aktivität nach G. festgestellt werden. Im Verlauf der gegenwärtigen Untersuchungen ließ sich kein Einfluß von G auf den 3H‐Thymidin‐Einbau in DNA von Hodenzellen beobachten. Auch ergaben sich keine Veränderungen des Gehaltes an DNA und Total‐Protein des Hodens. Plasma‐Na+ wurde nicht verändert. Ein flüchtiger Abfall des Plasma‐K+ ergab sich innerhalb von 2 Wochen Anwendung, während nach 4 Wochen ein Normalstatus wieder erreicht war. Die Hodenhistologie war erst nach 4 Wochen G‐Therapie deutlich beeinflußt im Sinne einer Spermatogenese‐hemmung. Die Motilität der Spermatozoen aus dem vas deferens war ebenfalls deutlich nach 4 Wochen herabgesetzt. Es wird festgestellt, daß aufgrund der gegenwärtigen Forschungsergebnisse ganz klar der Nachweis geführt werden kann, wonach Gossypol direkt die Spermatozoen beeinflußt und wonach Gossypol die ATPase‐Aktivität störend beeinflußt.

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Forty-four species of the genus Bruchidius have so far been collected from the different States in India during the period from May 1, 1974 to September 30, 1979 that the present project was in progress. Of these there are 21 species which appear to be new whereas the remaining 23 species have already been described. A list with the names of the species, their host-plants and the localities from where they have been collected is given, indicating the host-plant specificity and the transitional host-plants. A table showing Sexual Dimorphism in the species is appended. The Incidence and Distribution of the species in the different States of India show their populations to be dense, medium or rare based on their numbers. A map of India showing the incidence and distribution of the new species of Bruchidius is included. Information in regard to Additional host-plants and Additional localities is added only in the cases of already known species, whereas detailed descriptions are given in the cases of new species. A new key to the species of Bruchidius has been prepared. The biology and life-histories of 4 common species of Bruchidius yiz. B. pygcwocidotiis Arora, B. mimosae Arora, B. angustifrons Schilsky and B. tephrosiae Arora have been dealt with in detail, followed by a Table showing their comparative biology.

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