An enterotoxin A subunit-specific radioactive probe was used to correlate expression of vct-1 or vct-2 with the presence of specific restriction fragments in the DNAs of several wild-type and recombinant Vibrio cholerae strains. The data are consistent with the conclusion that vct is a cholera toxin structural gene. We previously reported that the gene vct specifies the antigenic structure of cholera toxin (CT) and is located between met and trp on the Vibrio cholerae genetic map (14). The vet-I
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